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Microtiter Dish Biofilm Formation Assay
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Immunology and Infection
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JoVE Journal Immunology and Infection
Microtiter Dish Biofilm Formation Assay

Microtiter Dish Biofilm Formation Assay

DOI:
10.3791/2437-v

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03:57 min

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January 30, 2011

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1Microbiology and Immunology,Dartmouth Medical School

Chapters

  • 00:05Title
  • 00:54Growing Biofilms
  • 01:28Staining Biofilms
  • 02:44Quantifying the Biofilms
  • 03:23Quantification of Biofilms Formed by P. fluorescens Over Time
  • 03:37Conclusion

Summary

Automatic Translation

Automatic Translation

The assay describes a rapid means to measure early biofilm formation in bacteria and fungi. This method uses a microtiter plate as the substratum for microbial biofilm formation, and the biofilm is visualized using crystal violet strain. The assay provides either a qualitative or quantitative assay for early biofilm formation.

Tags

Microtiter DishBiofilm Formation AssayMicrobesSurfacesMedicalIndustrialNatural SettingsExtracellular MatrixArchitectureMacrocoloniesFluid-filled ChannelsResistance To Antimicrobial AgentsAntibioticsEarly StagesBacterial BiofilmsFungal Biofilm FormationStatic Batch-growth ConditionsMature BiofilmsFlow Cell SystemsInitiation Of Biofilm FormationFlagellaPiliAdhesinsCyclic-di-GMP Binding And MetabolismExtracellular Polysaccharide Production

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