C18 Cartridge
C18 Cartridge - Related Videos
03:31
A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E
0 Views •
2025
This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.
08:04
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
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Cited by 133 •
2016
We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.
08:12
Characterization, Quantification and Compound-specific Isotopic Analysis of Pyrogenic Carbon Using Benzene Polycarboxylic Acids (BPCA)
0 Views •
Cited by 40 •
2016
We present the benzene polycarboxylic acid (BPCA) method for assessing pyrogenic carbon (PyC) in the environment. The compound-specific approach uniquely provides simultaneous information about the characteristics, quantity and isotopic composition (13C and 14C) of PyC.
10:34
Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water
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Cited by 6 •
2016
A protocol about the characterization and application of five different passive sampling devices is presented.
09:34
Extraction and Characterization of Surfactants from Atmospheric Aerosols
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Cited by 26 •
2017
Methods are presented for the targeted extraction of surfactants present in atmospheric aerosols and the determination of their absolute concentrations and surface tension curves in water, including their Critical Micelle Concentration (CMC).
FAQs
Retention depends on relative polarity: relatively nonpolar analytes interact with the bonded octadecyl chains, while more polar matrix components are less strongly retained and can pass through or be removed during washing. This polarity-based selectivity separates target compounds from interfering sample constituents before instrumental analysis, helping prepare a cleaner fraction for subsequent measurement.
The porous silica particles provide the solid support and surface area for the bonded phase, while the octadecyl chains provide the hydrocarbon character responsible for hydrophobic interactions. Together, these components create a sorbent that can retain relatively nonpolar compounds from a liquid sample while allowing more polar constituents to remain in the passing liquid or wash fraction.
Washing removes components that do not need to remain on the sorbent, particularly more polar matrix constituents that are not strongly retained. Elution then uses a stronger organic solvent to release the compounds held by the C18 phase. Keeping these steps distinct supports selective cleanup and provides a collected fraction containing the retained compounds for analysis.
The workflow proceeds through conditioning, sample loading, washing, and elution. Conditioning prepares the sorbent, loading brings the liquid sample into contact with the bonded phase, and washing removes unwanted matrix components. Finally, a stronger organic solvent elutes the retained compounds, producing a fraction suitable for the next analytical step.
Chemists would choose a C18 Cartridge when a liquid sample contains relatively nonpolar compounds mixed with more polar matrix components and the sample requires cleanup before analysis. The cartridge can isolate and concentrate the compounds of interest, making the resulting sample more compatible with chromatography or another chemical measurement method.
By removing portions of the sample matrix and concentrating retained compounds, the preparation can improve compatibility between the sample and the analytical method. The cleaned, concentrated fraction may also enhance sensitivity in chromatography and other analytical techniques. Its value therefore lies not only in separation, but also in preparing analytes for more effective chemical measurement.