Lc/ms Spectra
Lc/ms Spectra - Related Videos
02:39
Emission Spectra
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2020
When solids, liquids, or condensed gases are heated sufficiently, they radiate some of the excess energy as light. Photons produced in this manner have a range of energies, and thereby produce a continuous spectrum in which an unbroken series of wavelengths is present. In contrast to continuous spectra, light can also occur as discrete or line spectra having very narrow linewidths interspersed throughout the spectral regions. Exciting a gas at low partial pressure using an electrical current,...
09:40
Measurement and Analysis of Atomic Hydrogen and Diatomic Molecular AlO, C2, CN, and TiO Spectra Following Laser-induced Optical Breakdown
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Cited by 9 •
2014
Time-resolved atomic and diatomic molecular species are measured using LIBS. The spectra are collected at various time delays following the generation of optical breakdown plasma with Nd:YAG laser radiation and are analyzed to infer electron density and temperature.
07:51
Method for Recording Broadband High Resolution Emission Spectra of Laboratory Lightning Arcs
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2019
Emission spectroscopy techniques have traditionally been used to analyze inherently random lightning arcs occurring in nature. In this paper, a method developed to obtain the emission spectroscopy from reproducible lightning arcs generated within a laboratory environment is described.
06:28
Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
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Cited by 9 •
2017
Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.
07:38
Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
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Cited by 8 •
2025
Biological samples have distinct optical characteristics in the short-wave infrared (SWIR) compared to the visible (VIS) and near-infrared (NIR) wavelength ranges. However, recording pure SWIR absorption spectra is challenging and rarely conducted for biological molecules. This article presents a method to characterize the VIS-SWIR absorption spectra of biological absorbers.
FAQs
Liquid chromatography reduces overlap by separating compounds according to their interactions with the mobile and stationary phases. As components elute at different times, the mass spectrometer can examine their signals more selectively. This separation helps distinguish compounds with similar or related mass-to-charge values and supports more reliable characterization within chemically complex samples.
The mass-to-charge ratio, written as m/z, places detected ions along the spectrum’s mass axis. Its values can support molecular-mass assessment, while the relative ion intensities show which signals are more prominent. Interpreting m/z together with isotope patterns gives additional evidence for distinguishing and characterizing chemical compounds.
Isotope patterns provide a characteristic distribution of related signals that can support molecular-mass interpretation. Fragmentation products add structural information by showing smaller pieces formed from an ionized compound. Considering both types of evidence gives chemistry researchers more support for identifying a compound than relying on a single spectral signal alone.
A sample is first introduced to liquid chromatography, where its components separate through interactions with the mobile and stationary phases. Eluting compounds then enter the mass spectrometer, become ionized, and are recorded according to m/z and ion intensity. Researchers interpret the resulting signals, isotope patterns, and fragments to characterize the mixture’s compounds.
For qualitative analysis, researchers examine molecular-mass information, isotope patterns, and fragmentation products to help identify compounds. For quantitative analysis, the recorded ion signals provide measurement-related information about components in a sample. Applying both perspectives allows a single LC/MS investigation to address what compounds are present and support assessment of their amounts.
LC/MS spectra are applied to pharmaceuticals, metabolites, environmental contaminants, and other chemical mixtures. In these settings, the combined separation and detection information helps researchers characterize compounds that occur alongside many other components. The approach is therefore relevant to pharmaceutical analysis, metabolic investigations, environmental chemistry, and broader mixture analysis.