C Elegans Microinjection
C Elegans Microinjection - Related Videos
03:59
Gonad Microinjection: A Method of Compound Delivery Directly into the Germline of C. elegans
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2023
This video describes microinjection, a common method of creating transgenic C. elegans strains. In the example, we will see microinjection used to introduce a ribonucleoprotein (RNP) complex for CRISPR-bases gene editing.
12:09
Generation of Stable Transgenic C. elegans Using Microinjection
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Cited by 93 •
2008
This video demonstrates the technique of microinjection into the gonad of C. elegans to create transgenic animals.
11:34
Basic Caenorhabditis elegans Methods: Synchronization and Observation
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Cited by 452 •
2012
The easiness of maintaining and propagating the nematode C. elegans make it a nice model organism to work with. The possibility of synchronizing worms allows the work with a significant amount of subjects at the same developmental stage, what facilitates the study of one particular process in many animals.
10:54
C. elegans Maintenance
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2023
Ceanorhabditis elegans has been, and is still, used to great success as a model organism for studying a variety of developmental, genetic, molecular and even physical phenomena. In order to use C. elegans to its full potential, proper care and attention to the basic maintenance of this powerful organism is essential. In this video you will learn the basic housing and feeding requirements of C. elegans, how to correctly handle and manipulate worms using a worm pick and how to freeze and recover...
08:12
Zebrafish Microinjection Techniques
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2023
One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved either through direct injection into the blastomere, or via natural cytoplasmic movements that occur after a...
FAQs
The syncytial gonad is important because it provides access to germline material while developing oocytes share a common cytoplasmic environment. Introducing reagents there allows the delivered genetic material to influence progeny, rather than limiting the manipulation to an individual somatic tissue. This connection explains the technique’s value for studying inherited genetic effects.
Injected DNA can assemble into heritable extrachromosomal arrays, providing a route to transgene expression and reporter production without requiring a targeted change at a defined sequence. By contrast, a CRISPR-based mixture is used when the goal is a targeted sequence change. This distinction helps researchers match the reagent strategy to the genetic question.
DNA, RNA, and genome-editing reagents serve different experimental purposes in the injected mixture. DNA supports introduced constructs such as transgenes or fluorescent reporters, whereas RNA and genome-editing reagents enable other forms of gene manipulation, including CRISPR-based targeting. Selecting among them therefore depends on whether the experiment emphasizes expression, rescue, or sequence modification.
A heritable outcome extends the experiment beyond the injected adult and allows genetic effects to be evaluated in progeny. Extrachromosomal arrays provide one route for inherited transgene expression, while CRISPR-based mixtures can support inheritance of targeted sequence changes. Consequently, examining progeny helps distinguish transient delivery from outcomes useful for genetic analysis.
An adult hermaphrodite is immobilized, a fine needle is used to penetrate the syncytial gonad, and the selected genetic material is delivered directly into that compartment. The resulting progeny can then be examined for inherited extrachromosomal arrays or targeted sequence changes, depending on the reagent mixture and experimental objective.
The relevant injected materials are DNA, RNA, or genome-editing reagents. DNA may generate heritable extrachromosomal arrays, while CRISPR-based mixtures can support targeted sequence changes. This choice also shapes the type of readout: researchers may focus on transgene expression and reporters, gene rescue, or altered sequence function.
In genetics, the method supports transgene expression, fluorescent reporter creation, gene rescue, and functional analysis. It can also test regulatory elements in vivo, linking a DNA sequence to its effects within the organism. These applications make the approach useful for rapid evaluation of gene function and for connecting sequence changes with observable genetic outcomes.