Flpe Recombinase
Flpe Recombinase - Related Videos
12:02
Molecular Evolution of the Tre Recombinase
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Cited by 4 •
2008
Here we report the generation of Tre recombinase through directed, molecular evolution. Tre recombinase recognizes a pre-defined target sequence within the LTR sequences of the HIV-1 provirus, resulting in the excision and eradication of the provirus from infected human cells. While still in its infancy, directed molecular evolution will allow the creation of custom enzymes that will serve as tools of molecular surgery and molecular medicine.
07:06
Principles of Site-Specific Recombinase (SSR) Technology
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Cited by 14 •
2008
The advent of site-specific recombinase (SSR) technology and the Cre/lox system has led to numerous advances in molecular biology, and has proven itself as a valuable tool for assessing gene function in transgenic animals. This interview discusses the mechanism of site specific recombination by Cyclization recombinase (Cre) and how the use of this enzyme has led to the development of conditional mutagenesis, which has significant advantages over traditional knock out strategies.
10:20
Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
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Cited by 2 •
2008
Current HIV-1 strategies act to suppress the viral life cycle but do not effectively eradicate infection. Here, we demonstrate that an engineered recombinase can efficiently excise integrated HIV-1 proviral DNA from the genome of infected cells.
12:13
TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
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Cited by 37 •
2012
A quick method to modify the genome of V. cholerae is described. These modifications include the deletion of single genes, gene clusters and genomic islands as well as the integration of short sequences (e.g. promoter elements or affinity-tag sequences). The method is based on the natural transformation and FLP-recombination.
03:12
CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model
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2025
This video describes a procedure for target gene knockout using a tamoxifen-inducible Cre-recombinase system following intraperitoneal injection of tamoxifen in a mouse model.
FAQs
The relative orientation of paired FRT sites determines the rearrangement. Sites aligned in the same direction permit removal of the intervening DNA, while sites facing opposite directions support inversion of that segment. This orientation-dependent behavior lets investigators design distinct genome-engineering outcomes, including targeted deletion or reversal of a defined genetic sequence.
Paired FRT sites provide the defined DNA landmarks that guide the recombination event. Their placement determines which sequence is altered and how the rearrangement occurs, helping restrict the genetic change to a selected region rather than producing an unspecified genome-wide effect. This specificity is central to controlled biomedical experiments.
Flpe recombinase enables genetic changes to be controlled by where and when recombination occurs. When appropriately positioned FRT sites surround a relevant sequence, recombination can support conditional gene activation or deletion. This control allows researchers to connect a gene’s function with a particular biological setting and examine resulting phenotypes more precisely.
Deletion and inversion produce different consequences even though both depend on FRT-site arrangement. Same-orientation sites allow the intervening sequence to be excised, removing it from the relevant genetic configuration. Opposite-orientation sites reverse that sequence instead. Selecting between these outcomes lets investigators test whether gene behavior depends on sequence presence or orientation.
In transgenic animal models, Flpe recombinase provides a way to introduce defined genetic rearrangements for studying gene function. Investigators can use conditional activation or deletion to connect a selected genetic change with an observed phenotype. These models are especially useful when disease biology requires analysis of gene effects under controlled biological conditions.
Flpe recombinase can help mark or alter defined genetic sequences in ways that support lineage tracing, allowing researchers to relate genetic history to later biological outcomes. In disease studies, the resulting conditional models help test gene function, clarify links between genes and phenotypes, and evaluate whether a gene represents a potential therapeutic target.