Pulmonary Metabolism
Pulmonary Metabolism - Related Videos
08:17
Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery
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Cited by 8 •
2017
The following protocol describes the methodology for the acquisition and analysis of echocardiographic images used to obtain the Left Atrial Volume (LAV), Aorta (Ao) diameter, and Pulmonary Artery (PA) diameter in mice. This technique is a non-invasive, non-terminal procedure that allows assessment of the cardiopulmonary function.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
06:59
Catheter-based Endovascular Angioplasty for Fibrosing Mediastinitis-associated Pulmonary Vein Stenosis
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2025
Pulmonary vein stenosis (PVS) is a life-threatening complication of fibrosing mediastinitis (FM), with a poor prognosis if not properly treated. Currently, catheter-based endovascular angioplasty is a promising therapeutic strategy for FM-associated PVS. This protocol primarily outlines a stepwise approach to the transcatheter procedure for FM-associated PVS.
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
Cytochrome P450s, esterases, and monoamine oxidase provide distinct biochemical routes for modifying compounds in lung tissues. Depending on the compound and reaction, these enzymes may activate it, inactivate it, or otherwise alter its structure. That distinction matters because an exposure can produce either a desired local effect, reduced activity, or a toxic product before broader distribution.
Epithelial, endothelial, and resident immune cells are all potential sites of pulmonary metabolic activity, but they occupy different airway or vascular compartments. Consequently, the same compound may encounter different enzymatic environments as it moves through lung tissues. This cellular and anatomical context helps explain why metabolism can produce effects within the lung while also changing the amount that enters systemic circulation.
By transforming inhaled substances before they reach the circulation, the lung can alter the fraction that becomes systemically available. This may limit systemic exposure, change systemic effects when activation occurs, or generate local toxicity that circulating concentrations alone would not predict. Pulmonary metabolism therefore connects respiratory exposure with pharmacokinetic behavior and safety assessment.
Inhaled drug design can account for whether lung enzymes are likely to activate or inactivate a candidate and whether local transformation may create toxicity. These considerations connect intended respiratory action with systemic exposure, rather than treating the inhaled compound as chemically unchanged. The same framework supports pharmacokinetic modeling of movement through airway and vascular tissues.
Pulmonary metabolism helps explain how airborne chemicals and pharmaceutical exposures produce local lung effects or influence the body after entering the respiratory system. Toxicology can examine whether enzymatic transformation changes a compound’s activity and can consider consequences within lung tissues alongside systemic exposure. This perspective is especially relevant when evaluating chemicals that pass through airway or vascular compartments.
It supports research into inhaled drug behavior, toxicology, pharmacokinetic modeling, and lung diseases affected by environmental or pharmaceutical exposures. Studies can relate enzyme activity in epithelial, endothelial, and resident immune cells to compound transformation and movement through lung tissues. The resulting context helps investigators interpret local respiratory effects together with changes in systemic exposure.