Thrombus Volume Quantification
Thrombus Volume Quantification - Related Videos
09:01
Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse
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Cited by 4 •
2017
The ear model of the hairless SKH1-Hrhr mouse enables intravital fluorescence microscopy of microcirculation and phototoxic thrombus induction without prior surgical preparation in the examined microvascular bed. Therefore, the ear of the hairless mouse is an excellent in vivo model to study the complex interactions during microvascular thrombus formation, thrombus evolution, and thrombolysis.
05:54
Intravital Fluorescence Microscopy to Study Microvascular Thrombus Formation
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2025
This video demonstrates the intravital fluorescence microscopy technique to study thrombus formation in a mouse model. The mouse is injected with a fluorophore-labeled polysaccharide to monitor blood circulation. Upon illumination with a high-intensity light, the phototoxic induction of an injury in the microvasculature of the ear leads to the formation of a thrombus, which is confirmed by visualizing the obstruction in the blood flow.
08:50
Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
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2026
This work describes a microfluidic assay that uses shear stress to trigger thrombus formation in blood and characterizes the thrombus by multiple dimensions of readout. The assay can measure the prothrombotic tendency of blood samples, and is thus useful in disease diagnosis, drug discovery, as well as basic mechanistic studies related to thrombosis.
02:05
Visualization of a Cerebral Thrombus in a Mouse Using Micro-Computed Tomography Imaging
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2025
Source: Kim, D., et. al. Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli. J. Vis. Exp. (2016)This video demonstrates visualization of cerebral thrombus in mice using micro-computed tomography (micro-CT) imaging. A cerebral thrombus mouse model is injected with fibrin-targeted gold nanoparticles acting as a contrasting agent. The mouse is placed on the bed of a micro-CT machine, and images are acquired from multiple angles.
11:18
Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
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Cited by 29 •
2013
Here we report an experimental technique of fluorescence intravital microscopy to visualize heterotypic platelet-neutrophil interactions on the activated endothelium during vascular inflammation and thrombus formation in live mice. This microscopic technology will be valuable to study the molecular mechanism of vascular disease and to test pharmacologic agents under pathophysiological conditions.
FAQs
Image scale and slice thickness convert segmented image regions into physical volume. The calculation uses the dimensions represented by pixels or voxels and the spacing between sequential slices, rather than simply counting image elements. This calibration makes measurements more meaningful when thrombus burden is compared across scans, patients, or studies, particularly when acquisition dimensions differ.
Boundary identification determines which image regions belong to the clot and therefore which regions enter the volume calculation. Consistent segmentation across sequential slices or image voxels helps ensure that the summed regions represent the same thrombus extent. This consistency strengthens comparisons of clot burden, treatment response, and outcomes across imaging assessments.
Three-dimensional analysis captures the clot's extent across multiple image planes instead of relying on a single cross-sectional view. By combining segmented regions from sequential slices or voxels, the method produces a volume that better represents overall thrombus burden. That measure can support clot characterization and evaluation of how burden relates to clinical risk or therapeutic effectiveness.
A measurement workflow begins by selecting vascular images from CT, MRI, or another applicable imaging workflow. The thrombus boundary is identified and segmented across sequential slices or image voxels, after which the enclosed regions are summed. Image scale and slice thickness are incorporated so the reported result represents physical volume for later comparison or interpretation.
Researchers may use the measurement to characterize a clot, support treatment planning, monitor changes over time, or compare outcomes between patients or studies. Repeated imaging can provide a quantitative basis for assessing treatment response, while comparisons of thrombus burden can help investigate relationships between clot size, clinical risk, and therapeutic effectiveness.
Longitudinal studies can use repeated volume measurements to track thrombus burden across imaging time points. Applying the same boundary-based segmentation and accounting for image scale and slice thickness allows observed differences to be interpreted as changes in measured clot volume. The resulting data can help evaluate treatment response and compare disease-related outcomes over time.