Katanin 60
Katanin 60 - Related Videos
08:40
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Cited by 1 •
2018
Here we present a protocol for the fabrication of C60/graphene hybrid nanostructures by physical thermal evaporation. Particularly, the proper manipulation of deposition and annealing conditions allow the control over the creation of 1D and quasi 1D C60 structures on rippled graphene.
08:38
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Cited by 8 •
2016
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03:55
Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium
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Cited by 2 •
2023
This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.
09:38
Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
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Cited by 6 •
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Scalable engineered blood vessels would improve clinical applicability. Using easily sizable 3D-printed guides, rings of vascular smooth muscle were created and stacked into a tubular form, forming a vascular graft. Grafts can be sized to meet the range of human coronary artery dimensions by simply changing the 3D-printed guide size.
11:04
Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
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Cited by 4 •
2013
This study used a multi-well plate microfluidic system, significantly increasing throughput of cell rolling studies under physiologically relevant shear flow. Given the importance of cell rolling in the multi-step cell homing cascade and the importance of cell homing following systemic delivery of exogenous populations of cells in patients, this system offers potential as a screening platform to improve cell-based therapy.
FAQs
ATP hydrolysis provides the energy required for Katanin 60 to engage tubulin in the microtubule lattice and destabilize a localized region. This energy-dependent action allows the complex to sever an existing microtubule rather than merely leave the polymer unchanged. The resulting fragments can then be reorganized or regrown, linking chemical energy use to cytoskeletal remodeling.
Lattice engagement positions Katanin 60 directly on the microtubule structure that must be remodeled. By acting on tubulin within that lattice, the protein promotes local destabilization and produces a break at a defined region. This mechanism gives cells a way to alter microtubule length and arrangement, rather than relying only on whole-polymer growth or loss.
Severing converts existing microtubules into shorter fragments that can be reorganized or regrown. In neurons, this creates a mechanism for adjusting the cytoskeletal layout as cellular regions change shape or extend. Regulated remodeling is especially relevant to neuronal polarization, axon extension, and dendrite branching, where microtubule organization must remain coordinated with complex cell architecture.
The activity is relevant to neuronal polarization, axon extension, and dendrite branching, as well as other processes requiring precise cytoskeletal remodeling. These events depend on changing the organization of microtubules while neuronal processes become established or elaborated. Studying Katanin 60 therefore connects a molecular severing mechanism with the formation and maintenance of diverse neuronal structures.
Research on Katanin 60 can clarify how neurons shape and maintain long, structurally complex processes. Its activity provides a molecular framework for examining how cytoskeletal remodeling supports neuronal form and extension. This information may also illuminate mechanisms associated with neurodevelopmental and neurodegenerative disorders, particularly when microtubule organization or remodeling is implicated.
Axons and dendrites are neuronal processes whose growth and branching depend on controlled cytoskeletal remodeling. Katanin 60 contributes to this context by promoting microtubule severing, generating fragments that can be reorganized or regrown as these structures develop. Examining its activity across both compartments helps relate one remodeling mechanism to the distinct architectural demands of complex neurons.