Tissue Sample Analysis
Tissue Sample Analysis - Related Videos
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
08:48
Quantitation of γH2AX Foci in Tissue Samples
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Cited by 7 •
2010
Quantitation of DNA double-strand breaks on the basis of γH2AX foci has become an invaluable tool, particularly in radiation biology, for the evaluation of tissue radiosensitivity and effects of radiation modifying compounds. Here we demonstrate the use of an immunofluorescence assay for quantitation of γH2AX foci in tissue samples.
10:16
Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
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Cited by 186 •
2016
The protocol below describes the methodology for: microplastics sampling on the sea surface, separation of microplastic and chemical identification of particles. This protocol is in line with the recommendations for microplastics monitoring published by the MSFD Technical Subgroup on Marine Litter.
02:08
A Hydrophobic Tissue Clearing Method for a Rat Brain Tissue Sample
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2025
Source: Kirchner, K.N., et al., A Hydrophobic Tissue Clearing Method for Rat Brain Tissue. J. Vis. Exp. (2020).This video demonstrates the method of hydrophobic tissue clearing of a rat brain tissue sample. The sample is sequentially dehydrated in methanol, treated with a dichloromethane and methanol mixture to dissolve lipids and pigments, and transferred to dibenzyl ether for complete tissue clearing, enabling detailed imaging without thin sectioning.
05:45
Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples
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Cited by 2 •
2024
This protocol describes a technique for the analysis of respiratory supercomplexes when only small amounts of samples are available.
FAQs
Preservation is essential because it maintains brain or nervous tissue in a condition suitable for later examination. Researchers then prepare thin sections so internal organization can be viewed rather than hidden within an intact sample. These early steps affect whether neuronal arrangement, cellular responses, or pathological changes can be localized reliably in subsequent analyses.
Stains and molecular labels answer different but complementary questions. Stains make tissue features visible for examining overall structure, whereas molecular labels identify particular proteins or other components within the sample. Combining these approaches can connect where a molecular feature occurs with the surrounding cellular organization, helping relate tissue composition to changes in nervous system function.
Microscopy provides spatial information about prepared tissue sections. It can reveal the location of cells, proteins, and other tissue components, while related assays can add molecular information. This spatial readout is valuable when researchers need to compare neuronal organization or pathology with localized cellular and molecular changes in the nervous system.
An important interpretive goal is to connect observations at several biological levels. A change in tissue structure may be considered alongside altered composition, protein localization, or cellular responses rather than treated as an isolated finding. In neuroscience, this integrated view helps relate microscopic evidence to nervous system function and processes such as injury or neurodegenerative disease.
Each stage prepares the sample for the next type of observation. Preservation keeps brain or nervous tissue suitable for examination; thin sectioning exposes internal regions; stains or molecular labels make selected features detectable; and microscopy or related assays record their distribution. Coordinating these steps allows structural and molecular observations to be interpreted within the same tissue context.
Tissue sample analysis is useful when a study needs evidence from nervous tissue at the structural, cellular, or molecular level. Supported applications include examining development, injury, neurodegenerative disease, and treatment effects. The resulting observations can show neuronal organization, pathology, or cellular responses, helping researchers connect biological changes with nervous system function.