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Метаболический маркировки Недавно Transcribed РНК за большой размер профилирования экспрессии генов синтеза РНК, производство и разложение в культуре клеток
Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
JoVE Journal
Биология
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JoVE Journal Биология
Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

Метаболический маркировки Недавно Transcribed РНК за большой размер профилирования экспрессии генов синтеза РНК, производство и разложение в культуре клеток

English

Сгенерировано автоматически
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27,020 Views

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11:00 min

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August 08, 2013

DOI:

10.3791/50195-v

DOI:
10.3791/50195-v

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11:00 min
August 08, 2013

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, , , , ,
1Max von Pettenkofer Institute, 2Отдел медицины,University of Cambridge, 3Institute for Informatics,Ludwig-Maximilians-University Munich

Резюме

Automatically generated

Всего клеточной РНК обеспечивает плохое шаблон для изучения краткосрочных изменений в синтез РНК и распада, а также кинетики процессинга РНК. Здесь мы описываем метаболический маркировки вновь транскрипции РНК с 4-тиоуридина последующим тиол-специфический биотинилирования и очистки вновь транскрипции РНК позволяющих преодолеть эти недостатки.

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Цитировать это СТАТЬЯ

Rädle, B., Rutkowski, A. J., Ruzsics, Z., Friedel, C. C., Koszinowski, U. H., Dölken, L. Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture. J. Vis. Exp. (78), e50195, doi:10.3791/50195 (2013).

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