Анализ in vitro для оценки влияния иммунорегуляторных Пути на ВИЧ-специфических CD4 Т-клеток эффекторной функцией

1:27

Depletion and Isolation of PBMCs via Ficoll Separation

2:08

Antibody Blockade and Antigen-Specific Stimulation of Cells

2:46

Collection and Analysis of Supernatant

3:15

Collection and Phenotypic Analysis of Cells via Flow Cytometry

4:09

Analysis of Cells via qRT-PCR

5:15

Adaptation for Intracellular Cytokine Staining at 48 Hours

5:48

Adaptation for Sorting Cells

6:55

Results: HIV-specific CD4 T Cell Effector Function

9:03

Conclusion