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Быстрое выявление грамотрицательных бактерий из крови культуральной жидкости Использование MALDI-TOF масс-спектрометрии
Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
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JoVE Journal Иммунология и инфекции
Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Быстрое выявление грамотрицательных бактерий из крови культуральной жидкости Использование MALDI-TOF масс-спектрометрии

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Сгенерировано автоматически
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24,253 Views

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13:29 min

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May 28, 2014

DOI:

10.3791/51663-v

DOI:
10.3791/51663-v

•

13:29 min
May 28, 2014

•

17 Views
, , , , ,
1Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research,Westmead Hospital, 2Centre for Research Excellence in Critical Infection, Westmead Millennium Institute,Westmead Hospital, 3Sydney Emerging Infectious Diseases Institute, University of Sydney,Westmead Hospital

Резюме

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Применение матрицы-лазерной десорбцией / время ионизации полета (MALDI-TOF) масс-спектрометрии (MS) непосредственно к культуре крови бульона ускоряет идентификацию бактерий. Представленный метод является быстрым и надежным методом идентификации грамотрицательных бактерий непосредственно из культуры крови бульоне.

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Rapid IdentificationGram Negative BacteriaBlood Culture BrothMALDI-TOF Mass SpectrometryClinical MicrobiologyBacterial IdentificationBloodstream InfectionSub-cultureSolid AgarMALDI-TOF MSBlood Culture SpecimensInterfering ResinsProteinsHemoglobinBacterial ConcentrationBacterial PurificationBacterial Extraction

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Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

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Цитировать это СТАТЬЯ

Gray, T. J., Thomas, L., Olma, T., Mitchell, D. H., Iredell, J. R., Chen, S. C. A. Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry. J. Vis. Exp. (87), e51663, doi:10.3791/51663 (2014).

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