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Генома белок-белковых Взаимодействие Скрининг белком-фрагментом комплементации анализа (РСА) в живых клетках
Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
JoVE Journal
Биология
This content is Free Access.
JoVE Journal Биология
Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Генома белок-белковых Взаимодействие Скрининг белком-фрагментом комплементации анализа (РСА) в живых клетках

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08:38 min

March 03, 2015

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08:38 min
March 03, 2015

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Proteins interact with each other and these interactions determine in a large part their functions. Protein interaction partners can be identified at high-throughput in vivo using a yeast fitness assay based on the dihydrofolate reductase protein-fragment complementation assay (DHFR-PCA).

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