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February 16, 2015
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This article describes a yeast growth-based assay for the determination of genetic requirements for protein degradation. It also demonstrates a method for rapid extraction of yeast proteins, suitable for western blotting to biochemically confirm degradation requirements. These techniques can be adapted to monitor degradation of a variety of proteins.
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Watts, S. G., Crowder, J. J., Coffey, S. Z., Rubenstein, E. M. Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae. J. Vis. Exp. (96), e52428, doi:10.3791/52428 (2015).
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