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August 04, 2015
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We describe a technique for concurrently measuring force-regulated single receptor-ligand binding kinetics and real-time imaging of calcium signaling in a single T lymphocyte.
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Chen, Y., Liu, B., Ju, L., Hong, J., Ji, Q., Chen, W., Zhu, C. Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell. J. Vis. Exp. (102), e52975, doi:10.3791/52975 (2015).
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