Capillaroscopy is a non-invasive, efficient, relatively inexpensive and easy-to-learn methodology for directly visualizing capillaries in the microcirculation. However, only one publication to date describes the reliability of a complex software program available for quantitating capillaroscopy data. Here, we present a simple, reliable protocol for quantitating capillaries using a standardized algorithm.
Cheng, C., Lee, C. W., Daskalakis, C. A Reproducible Computerized Method for Quantitation of Capillary Density using Nailfold Capillaroscopy. J. Vis. Exp. (104), e53088, doi:10.3791/53088 (2015).