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Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
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Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Двунаправленная ретровирусная интеграция сайта Методология ПЦР и количественный анализ данных

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10,757 Views

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12:53 min

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June 14, 2017

DOI:

10.3791/55812-v

DOI:
10.3791/55812-v

•

12:53 min
June 14, 2017

•

10722 Views
, , , , , ,
1UCLA AIDS Institute,University of California at Los Angeles (UCLA)2Department of Microbiology, Immunology, & Molecular Genetics,University of California at Los Angeles (UCLA)3Departments of Biomathematics and Mathematics,University of California at Los Angeles (UCLA)4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups,Korea Research Institute of Bioscience and Biotechnology5Отдел медицины,University of California at Los Angeles (UCLA)6Department of Veterinary Biosciences, College of Veterinary Medicine,The Ohio State University (OSU)

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В этой рукописи описывается экспериментальная процедура и программный анализ для анализа двунаправленного интеграционного сайта, который может одновременно анализировать ДНК-перекрестный узел вверх и вниз по течению. Двунаправленные продукты ПЦР могут использоваться для любой последующей секвенирующей платформы. Полученные данные полезны для высокопроизводительного количественного сравнения интегрированных целей ДНК.

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Цитировать это СТАТЬЯ

Suryawanshi, G. W., Xu, S., Xie, Y., Chou, T., Kim, N., Chen, I. S. Y., Kim, S. Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow. J. Vis. Exp. (124), e55812, doi:10.3791/55812 (2017).

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