Инвазивная гемодинамическая оценка правой желудочковой системы и гипоксия-индуцированной легочной артериальной гипертензии у мышей

Overview

This protocol outlines an invasive hemodynamic assessment of the right ventricle and pulmonary artery in mice through an open-chest surgery approach. It allows for simultaneous evaluation of right ventricular and pulmonary artery pressures, making it particularly useful for pulmonary arterial hypertension (PAH) models.

Key Study Components

Area of Science

• Cardiovascular physiology
• Small animal surgery
• Hemodynamic assessment

Background

• Invasive hemodynamic assessments are crucial for understanding cardiovascular function.
• Open-chest surgery provides direct access to the heart and pulmonary artery.
• This method can yield valuable data for PAH research.
• Simultaneous measurement of ventricular and arterial pressures enhances data accuracy.

Purpose of Study

• To evaluate right ventricular and pulmonary artery pressures in mice.
• To establish a reliable protocol for hemodynamic assessment.
• To facilitate research in pulmonary arterial hypertension models.

Methods Used

• Preparation of surgical equipment and pressure transducer catheter.
• Open-chest surgical technique for access to the heart.
• Simultaneous measurement of right ventricular and pulmonary artery pressures.
• Use of a pressure volume system for data collection.

Main Results

• Successful simultaneous measurement of right ventricular and pulmonary artery pressures.
• Demonstrated reliability of the protocol for PAH model evaluations.
• Provided insights into hemodynamic changes in small animals.
• Validated the use of this method for future cardiovascular research.

Conclusions

• The open-chest surgery protocol is effective for hemodynamic assessments.
• It offers a comprehensive approach to studying cardiovascular function in mice.
• This method can advance research in pulmonary arterial hypertension.

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