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HIGH SCHOOL

Chemistry

Concept Videos

Analytical Chemistry

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Introduction to Molecular Spectroscopy

Measuring Fluorescence and Phosphorescence

Description

Fluorometers and spectrofluorometers are instruments used to measure molecular fluorescence. They do this by choosing specific excitation and emission wavelengths. The two instruments use different light sources and wavelength-selection methods.

A fluorometer uses absorption interference filters ...

Transcript

Fluorometers and spectrofluorometers are two instruments used to measure molecular fluorescence, each featuring a light source, filters or monochromators, sample cell, transducer, and signal processor.

Fluorometers use low-pressure mercury vapor lamps as the excitation source, employing absorption or interference filters to select excitat...

Tags

Fluorescence PhosphorescenceFluorometer SpectrofluorometerExcitation Emission WavelengthsMonochromator SelectionXenon Arc LampMercury Vapor LampPhosphorescence DetectionChopper TechniqueLiquid Nitrogen TemperaturesSolid Matrix Immobilization