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Biology

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Basic Biology

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Biology II: Mouse, Zebrafish, and Chick

Zebrafish Embryo Injection for Gene Studies

Description

Zebrafish embryo injection is a way to change gene activity in early development. Researchers deliver genetic material or knockdown constructs into blastomeres, the embryonic cells that sit on top of the yolk in a newly fertilized egg. The method can place material directly into the blastomere or into the yolk, where natural...

Transcript

Microinjection of zebrafish embryos allows researchers to deliver solutions directly into the developing animal, in order to study gene function and developmental dynamics. Embryos from the 1 to 4-cell stage are frequently used for injection. Because there are no membranes separating the cells and the yolk at this early stage...

Tags

Microinjection TechniqueEmbryo InjectionPipette PreparationAgarose ChamberYolk InjectionCytoplasmic InjectionmRNA OverexpressionMorpholino KnockdownTransgenic Zebrafish

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