Name: knee injury assessment
Uploaded: 2023-04-30T06:45:49.000Z
Duration: 12 min 1 s
Channel: JoVE

K12 Standards

new-york-state-science-learning-standards

New York State Science Learning Standards

High School

Life Science

Structure and Function

HS-LS1-3.

Knee Exam

1. Inspection
<ol>
	<li>Ask the patient to walk, and observe for limping and pain.</li>
	<li>Carefully compare both knees and look for the following:
	<ol>
		<li>Swelling or effusion: Suggested when the injured knee is visibly larger than the uninvolved knee. Swelling is defined as enlargement of the knee outside of the joint and is due to bleeding or excessive fluid in the soft tissues around the knee. Effusion, on the other hand, is due to bleeding or excessive fluid inside the knee joint. Palpating for effusion is often needed to determine whether a knee is enlarged due to swelling or a joint effusion.</li>
		<li>Ecchymosis: Bruising around the knee as seen in recent trauma.</li>
		<li>Quadriceps atrophy: Seen within a few weeks of a significant internal derangement, such as a ligament or cartilage tear.</li>
	</ol>
	</li>
</ol>
2. Palpation
<ol>
	<li>Palpation for swelling and effusion 
	Encircle your fingers around the kneecap to evaluate for swelling. Swelling in front of the kneecap suggests pre-patellar bursitis or infection, while swelling behind the kneecap suggests a knee joint effusion and likely significant internal derangement. The amount of effusion has some diagnostic significance and can be roughly graded from 0 to 3+ where 0 stands for no effusion, 1+ stands for the trace effusion as seen in osteoarthritis or old meniscal tear, 2+ stands for the moderate effusion as seen in posterior cruciate ligament (PCL) or meniscal tear, and 3+ stands for large effusion that can indicate anterior cruciate ligament (ACL) tear or patellar dislocation.</li>
	<li>Palpation for tenderness 
	Location of the pain can suggest which structures might be injured. 
	Have the patient in the supine position and palpate the following areas with your fingertips:
	<ol>
		<li>Tibial tubercle: Feel for the roughened protrusion on the anterior surface of the proximal tibia (below the front of the knee in the midline). It is the insertion site for the patellar tendon. Tenderness here suggests Osgood Schlatter&#39;s disease in a child or adolescent.</li>
		<li>Patella: Palpate with your fingertips over and around patella (the large bone in front of the knee joint). Tenderness or pain around or under the patella suggests patellofemoral pain; over the top of the patella suggests pre-patella bursitis; under the patella suggests patellofemoral pain or chondral injury. Tenderness or pain at the inferior pole of the patella suggests Synding-Larsen disease (patella apophysitis).</li>
		<li>Patellar tendon: Palpate the broad rope-like structure, which begins just above the tibial tubercle and extends to the lower pole of the patella. Tenderness here suggests tendonitis.</li>
		<li>Joint line: This can be felt along the side of the knee, both medial and lateral. Tenderness or pain at the joint line may suggest a meniscus tear or osteoarthritis.</li>
		<li>Medial aspect of the knee: Palpate the medial collateral ligament (MCL), extending from the medial femoral condyle to the proximal medial tibia and the pes anserine bursa, located just below the anterior medial joint line.</li>
		<li>Lateral aspect of the knee: Palpate the lateral collateral ligament (LCL), which extends from the lateral femoral condyle to the proximal lateral tibia and fibula and the iliotibial (IT) band, which runs from the hip along with lateral side of the leg and hip and attaches on the lateral tibia at Gerdy&#39;s tubercle.</li>
	</ol>
	</li>
</ol>
3. Range of Motion (ROM)
The ROM of the knee joint is generally assessed passively, with the patient lying supine. The knee should be checked for the following motions:
<ol>
	<li>Extension (0&#176;)
	<ol>
		<li>Ask the patient to fully straighten the leg. A lack of knee extension suggests mechanical block (often from a torn meniscus, loose body, or large effusion).</li>
		<li>To detect a more subtle lack of extension, have the patient lying prone with thighs supported by the end of an examination table, and observe for the difference in heel height.</li>
	</ol>
	</li>
	<li>Flexion (130&#176;): Ask the patient to lie supine and bend the knee as much as possible, bringing the heel as close to the buttocks as possible. Flexion can be limited by joint effusion or quadriceps spasm.</li>
	<li>Extensor Mechanism: Ask the patient to actively straighten the knee in the sitting position. It is essential to check for active knee extension to assess the integrity of the extensor mechanism (quadriceps muscle and tendon, patella, and tibial tubercle).</li>
	<li>Crepitus: Feel for crepitus (palpable grinding produced by motion) by placing the palm of your hand over the knee as the knee is either actively or passively flexed and then extended. This sign is rarely clinically significant unless associated with pain, effusion, or limitation of motion.</li>
</ol>
4. Ligament Testing
<ol>
	<li>Test the medial collateral ligament (MCL) and lateral collateral ligament (LCL) for pain and laxity.
	<ol>
		<li>Place the patient in the supine position.</li>
		<li>Passively extend (straighten) the patient&#39;s knee and then slightly flex it (to 20&#176;).</li>
		<li>Test the MCL by applying valgus stress by holding the patient&#39;s ankle with one hand, while pushing against the lateral side of the knee with your other hand
		<ol>
			<li>Score the MCL injury by pain and laxity. 
			Laxity to valgus stress applied with the knee in full extension suggests injury to the ACL and/or PCL, as well as the MCL. The ACL and PCL are taut when the knee is in full extension and should thus act to prevent valgus laxity in this position. Bending the knee to 20&#176; loosens these ligaments and allows isolated testing of the MCL.</li>
		</ol>
		</li>
		<li>Test the LCL by exerting a varus stress by holding the ankle with one hand, while pushing against the medial side of the knee with your other hand, also with the knee extended and then flexed to 20&#176;.
		<ol>
			<li>Use the same grading for an MCL injury to describe an injury to LCL. 
			The LCL is much less commonly injured than the MCL. If significant laxity to varus stress is noted, suspect more serious injury to the posterolateral corner of the knee. Such an injury may accompany a knee dislocation and warrants urgent orthopedic referral.</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>Evaluate the ACL by performing the following tests:
	<ol>
		<li>Lachman test: With the patient supine and the knee in 20&#176; of flexion (as described above), grasp the proximal tibia with one hand and apply an anteriorly directed force on the tibia while stabilizing the thigh with your other hand placed on the lateral side of the femur. This is the definitive exam to evaluate for an ACL tear.</li>
		<li>Anterior Drawer test: With the patient lying supine and the knee in 90&#176; of flexion, place your hands around the upper aspect of the tibia, with your thumbs in the front of the knee, and pull the tibia forward. The sensitivity of this exam is limited because with the knee flexed to 90&#176;, the collateral ligaments are taut and restrain anterior motion.</li>
		<li>Pivot shift: With the patient supine, grasp the leg by the foot and rotate the leg internally while flexing the knee past 20&#176; and applying a slight valgus stress (pushing outside of knee medially). Look for the tibia to shift forward. This test is helpful in assessing the secondary restraints of the knee joint, and will usually be positive only when the knee is very lax. This test is often painful, which limits its usefulness.</li>
	</ol>
	</li>
	<li>PCL testing
	<ol>
		<li>Posterior drawer test: position the patient and place your hands as for anterior drawer test and push the tibia posterior. Significant posterior displacement of the tibia suggests a PCL injury.</li>
		<li>Sag sign: position the patient supine with the knee flexed to 90&#176; and foot flat on exam table. Note if the tibia sags (drops back) with respect to the femur. This suggests a PCL tear with significant posterior laxity.</li>
	</ol>
	</li>
</ol>
5. Meniscal Tests
The tests done to evaluate for meniscal injury are often non-specific with a high rate of false positives. The most common meniscal tests described include:
<ol>
	<li>Bounce test: With the patient lying supine, force the knee into full extension by pushing down on the kneecap. An injured meniscus will cause significant pain as it gets pinched with knee extension.</li>
	<li>Joint line tenderness: Palpate along medial and lateral joint lines, where the meniscus is located. Tenderness here suggests injury to either the meniscus or osteoarthritis.</li>
	<li>Prone knee extension: Ask the patient to lie prone with both knees hanging just off the end of the exam table, and then look for a difference in heel height. This may indicate a mechanical block to knee extension caused by a torn and displaced meniscus.</li>
	<li>Duck walk: Ask the patient to get into a full squat and then walk in the squatted position. If able to do this, the patient is unlikely to have a significant cartilage or ligament injury.</li>
	<li>McMurray&#39;s test
	<ol>
		<li>With the patient lying supine, place one hand on the knee (providing varus stress) and the other hand on the heel.</li>
		<li>Perform the test by flexing and extending the patient&#39;s knee while internally and externally rotating the tibia. A significant clunk with this maneuver may indicate a displacing meniscal tear. This test should be done with caution as it may cause a torn meniscus to displace and lock the knee joint. The usefulness of this test is limited by its high rate of false positives.</li>
	</ol>
	</li>
	<li>Apley compression test
	<ol>
		<li>Have the patient lie prone on the examination table with the knee bent to 90&#176;.</li>
		<li>Passively flex and extend the patient&#39;s knee while rotating the tibia both internally and externally with one hand, and simultaneously apply an axial load on the knee joint by pushing down on the heel with the other hand.</li>
		<li>Watch for a significant clunk during this maneuver, which may indicate a torn meniscus. The utility of this test is also severely limited by a high false positive rate.</li>
	</ol>
	</li>
</ol>
6. Patella Tests
Several tests can be done to evaluate for patellofemoral dysfunction, pain, or dislocation. The patella tests are performed with the patient lying in the supine position.
<ol>
	<li>Apprehension test: Push the kneecap in a lateral direction. The test is positive when the patient becomes apprehensive that the patella may recurrently dislocate. This suggests a prior patella dislocation.</li>
	<li>Patella grind test: Push down on the kneecap and grind it back and forth. This test can aggravate the pain of patellofemoral dysfunction and has a very high false positive rate.</li>
	<li>Q-angle (quadriceps angle): With the patient lying supine, estimate the angle formed by a line connecting the anterior superior iliac spine (ASIS) of the hip and the center of the patella, with a line down the anterior tibia (from the center of the patella to the tibial tubercle). Angles greater than 10&#176; in male and 15&#176; in female predispose to patellofemoral problems. The wider pelvis seen in women increases the Q-angle and predisposes the patient to patellofemoral pain.</li>
</ol>

Examination of the knee is best done following a stepwise approach with the patient in sitting and supine positions. The patient should be observed for limping or pain while walking into and out of the exam room. It is important to have the patient remove enough clothing so that the surface anatomy of the knee can be seen and compared to the uninvolved side. The knee exam should begin with inspection, looking for asymmetry between the involved and uninvolved knee, noting whether the involved knee is larger (indicating...

knee exam

Source: <a href="https://www.jove.com/author/Robert+E._Sallis">Robert E. Sallis</a>, MD. Kaiser Permanente, Fontana, California, USA
The knee is a hinged joint that connects the femur with the tibia. It is the largest joint in the body, and due to its location in the middle of the lower leg, it is subjected to a variety of traumatic and degenerative forces. Examination of the knee can be quite complex, owing to the fact it is an inherently unstable joint held together by various ligaments and supported by menisci, which act as shock absorbers and increase the contact area of the joint. In addition, the patella lies in front of the knee, acting as a fulcrum to allow the forceful extension of the knee needed for running and kicking. As the largest sesamoid bone in the body, the knee is a common source of pain related to trauma or overuse.
When examining the knee, it is important to remove enough clothing so that the entire thigh, knee, and lower leg are exposed. The exam begins with inspection and palpation of key anatomic landmarks, followed by an assessment of the patient's range of motion (ROM). The knee exam continues with tests for ligament or meniscus injury and special testing for patellofemoral dysfunction and dislocation of the patella. The opposite knee should be used as the standard to evaluate the injured knee, provided it has not been previously injured.

Source: Robert E. Sallis, MD. Kaiser Permanente, Fontana, California, USA
The knee is a hinged joint that connects the femur with the tibia. It is the ...

Due to its location, the knee is subjected to a variety of traumatic and degenerative forces, and its intricate structure makes knee examination quite complex.Although the knee is the largest joint in the body, it has an inherently unstable structure held together by various ligaments, and supported by menisci, which increase the contact area of the joint, and act as shock absorbers. The patella, which is the largest sesamoid bone in the body, lies in front of the knee, attached to the quadriceps femoris tendon and the patella tendon. It acts as a fulcrum to allow forceful extension of the knee, which is needed for activities like running, making patella a common source of pain related to trauma or overuse.Here, we will review the essential steps required to carry out a comprehensive knee examination, combined with relevant anatomical illustrations. We will also explain the diagnostic relevance of various findings that one may come across during knee assessment.Like most musculoskeletal exams, let's begin with inspection and palpation. Before you start, make sure that the patient has removed enough clothing to expose their knees and lower legs. Ask the patient to walk back and forth, while you observe for limping, pain, swelling, effusion, ecchymosis and quad atrophy. Remember, if the patient has an injured knee then the non-injured knee should be used as the standard for evaluation.The next step is palpation. Remember to wash your hands thoroughly before you touch the patient. Ask the patient to sit on the exam table and then have them lay down supine. Start by assessing for swelling around the kneecap. Milk down from above and below the knee and then push down on the patella with your finger to appreciate any fluid above and below it. Repeat the same on the other side. Swelling in front of the kneecap suggests pre-patella bursitis or infection, whereas presence of fluid behind the patella suggests a knee joint effusion and likely significant internal derangement.Next, palpate the tibial tubercle for tenderness. This is the insertion site of the patella tendon. Bend the patient's knee and feel the roughened protrusion on the anterior surface of the proximal tibia. Tenderness at the tubercle may suggest Osgood Schlatter's disease in children and adolescent population. From there, palpate the patella tendon, which is broad rope-like structure beginning just above the tubercle and extending to the lower pole of the kneecap. Tenderness along the tendon indicates inflammation.From there, palpate the region around patella, this time checking for tenderness. Start at the inferior pole, then palpate the medial and lateral sides, and lastly feel the top of the kneecap. Tenderness at the inferior pole may suggest Synding-Larsen disease in children and adolescents, around or under may indicate patellofemoral pain, and over the top may suggest pre-patella bursitis.Next, feel the joint line. Put your finger and thumb on either side of the patella tendon, and then push along the lateral and medial side, checking for tenderness or pain at the joint line, which, if present, suggests a meniscus tear or osteoarthritis.Finally, palpate the medial and lateral aspect of the knee. Start at the medial femoral condyle, the attachment site of the medial collateral ligament, which extends from the condyle to the proximal medial tibia. Next, check the pes anserine bursa located just below the anterior medial joint line for tenderness. After that, palpate the lateral femoral condyle, the start site for the lateral collateral ligament, which can be felt till it inserts into to the proximal lateral tibia and fibula. Lastly, palpate the iliotibial band that runs from the hip, along the lateral side, and attaches on the tibia at Gerdy's tubercle.After the inspection and palpation portion of the exam is complete, move on to testing the knee's range of motion.While in supine position have the patient fully straighten their leg and lift it up slightly to test extension. Normally, this should reveal a 0? angle. Next, check for knee flexion. Passively, bend the patient's knee by bringing their heel as close to their buttocks as possible. The range for this motion should be about 130?. If limited, it can indicate joint effusion or quadriceps spasm.Next, have the patient transition into seated position and ask them to straighten their knee. This is to test the integrity of the extensor mechanism. Lastly, feel for crepitus -- the palpable grinding produced by motion -- by placing your palm over the knee, as it is either passively or actively, flexed and extended. This sign is rarely clinically significant, unless associated with pain, effusion, or limitation of motion.After performing all the above-mentioned maneuvers, evaluate the structure of the knee by using a variety of special tests. The first of these is the Ligament Testing. These maneuvers assess pain and laxity in the medial and lateral collateral ligaments, and the anterior and posterior cruciate ligaments, abbreviated as the MCL, LCL, ACL and PCL, respectively.For the MCL and LCL tests, first straighten the patient's knee by lifting it at the ankle with one hand, then use your other hand to flex it to about 20?. To assess the MCL, keep the knee in flexed position, and apply valgus stress by pushing the ankle outside. Then do the same with the knee extended. To check the LCL, switch hand positions, and apply varus stress by pushing the ankle medially with the knee extended and then flexed. In either case, score the ligament injury by pain and laxity as shown in this table.The next couple of tests, namely the Lachman test and the Anterior Drawer test examine the ACL. For the Lachman test, request the patient to relax their legs, then flex their knee to about 20? angle. And while pulling forward on the proximal tibia with one hand, push down on the femur with the other. With this maneuver, you are feeling for an end point as the ACL gets stressed.Next, conduct the Anterior Drawer Test. Ask the patient to bend their knee and lay their foot flat on the table. Then, sit on the foot so that it does not move. Place your hands just below the knee with both index fingers at the posterior joint line and pull to check if the tibia moves forward, which it should not.Following two maneuvers - the Posterior Drawer test and the looking for Sag Sign -evaluate the PCL. For the Posterior Drawer test, have the patient bend their knee just like the Anterior drawer test, then put both your thumbs on either side of the patella tendon, and pull back and push posteriorly on the upper aspect of the tibia. Significant displacement may suggest a PCL injury.Lastly, perform the Sag sign test. With the patient's knee flexed to 90? and foot flat on the exam table; note if the tibia sags or drops back with respect to the femur. It is a good approach to compare between sides. Presence of this sign may suggest a PCL tear with significant posterior laxity.After ligament testing, perform the following maneuvers that check for meniscal injury. However, one should keep in mind that these tests are often nonspecific and require additional confirmation.First of these tests, is the Bounce test. With the patient supine, lift the leg at the ankle and gently "bounce" the knee by forcing it into full extension. This maneuver will cause pain if the meniscus is torn. To assess the joint line tenderness palpate along the medial and lateral joint lines, where the meniscus is located. Any tenderness here may suggest either an injury to the meniscus or osteoarthritis.Next, ask the patient to transition into prone position with both knees hanging just off the end of the exam table. This is the prone knee extension test. Difference in heel height may indicate a mechanical block to knee extension caused by a torn and displaced meniscus.Subsequently, conduct the McMurray's test. First, bend the patient's knee and flex it fully, then internally rotate and extend it. Repeat the same by rotating it externally. A significant clunking sound during this test may indicate a displacing meniscal tear. This maneuver should be done with caution as one may cause a torn meniscus to displace and lock the knee joint.Next, perform the Apley Compression test. With the patient prone, passively flex the knee to 90?, rotate the tibia internally, apply axial load, and flex and extend the knee continually to check for any clunking sound. Repeat the maneuver while rotating the tibia externally. Significant clunking may indicate a torn meniscus.For the last meniscal test, called the Duck Walk Test. Request the patient to get into a full squat position and then walk. A patient is unlikely to have a significant cartilage or ligament injury if they are able to do this.Finally, a few tests called the patella tests can be performed to evaluate for patellofemoral dysfunction, pain, or dislocation.First maneuver in this group is called the apprehension test. Push the patient's kneecap in a lateral direction, and see if the patient becomes apprehensive that the patella may dislodge, which suggests a prior patella dislocation. Next, perform the patella grind test by pushing down on the patient's kneecap and grind it back and forth. Pain experienced by the patient during this maneuver may suggest patellofemoral dysfunction.Finally, check the quadriceps- or the Q-angle. With the patient supine, estimate the angle formed by a line connecting the anterior superior iliac spine and the center of patella, with a line from the center of patella down the anterior tibia to the tibial tubercle. Angles greater than 10? in males and 15? in females suggest a predisposition to patellofemoral problems.You have just watched a JoVE video detailing the knee assesment. In this video, we reviewed the essential aspects of this exam including inspection, palpation, range of motion testing, and additional special diagnostic maneuvers to narrow down the differential diagnosis associated with knee problems. As always, thanks for watching!

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Clinical Skills

Physical Examinations III

knee injury assessment

Knee injury assessment focuses on the largest joint in the body, where the femur meets the tibia. Because the knee sits in the middle of the lower leg, it faces traumatic and degenerative forces. Its structure also makes the exam more detailed. The joint is inherently unstable and relies on ligaments and menisci for support.

The menisci act as shock absorbers and help increase the contact area of the joint. The patella sits in front of the knee and acts as a fulcrum for forceful extension. That movement is important for actions like running and kicking. The patella is also the largest sesamoid bone in the body, so the knee is often a source of pain after trauma or overuse.

A proper knee exam starts with enough clothing removed to expose the entire thigh, knee, and lower leg. The clinician first inspects and palpates, which means looking at and feeling key anatomic landmarks. Next, the examiner checks range of motion, or ROM. The exam then moves to tests for ligament or meniscus injury and special tests for patellofemoral dysfunction and dislocation of the patella.

The opposite knee is often used as the standard for comparison. This works best when the other knee has not been injured before. Using the healthy side helps the examiner judge changes in the injured knee more accurately.

Knee Injury Assessment

cranial nerves exam i (i-vi)

Cranial Nerve Exam: Eye and Face Checks

Cranial nerve testing uses careful observation to check how the eyes, face, and related muscles are working. Some problems are easy to spot. A patient may report loss of smell or double vision, and facial nerve palsy can also be seen on exam. Other issues, such as sixth nerve palsy, may be subtle and need a careful neurological exam to find them. Cranial nerve problems can also appear when a person has changes in mental status. Some neurodegenerative disorders and brain lesions can affect...

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Cranial nerves VII-XII are tested as part of the neurological exam after the mental status evaluation. The examination begins with simple observations made during the first greeting. Facial weakness may already be visible, since the facial muscles are controlled by cranial nerve VII. Cranial nerve VII is the facial nerve. It also carries taste from the anterior two-thirds of the tongue and part of the external auditory canal. If facial weakness is paired with taste loss on the same side, that...

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Motor Function Assessment Basics

Motor function assessment begins with careful observation. Abnormal motor findings can appear in many diseases, including movement disorders, myopathies, and strokes. The clinician first watches how the patient walks into the exam area. They also note walking without help, speed, and coordination while moving. Patient history gives another chance to look for signs such as tremors. It can also reveal other abnormal movements, including chorea and tardive dyskinesia. These early observations may...

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Neurological Reflexes, Coordination, and Gait

Neurological motor exams often include testing reflexes, coordination, and gait. These parts of the exam help localize neurologic problems and show how the nervous system is working. Reflex testing includes deep tendon reflexes and superficial reflexes. A deep tendon reflex, or DTR, begins when a stretch-sensitive receptor in a neuromuscular spindle is stimulated. That signal travels through a single synapse to a motor nerve and produces a muscle contraction. DTRs are often increased in...

sensory exam

Sensory Pathways and Nerve Mapping

A sensory exam checks pain, temperature, light touch, vibration, and joint position sense. It helps localize nerve and brain problems by showing which sensory pathways are affected. Sensation from the face is covered in Cranial Nerves Exam I and II, along with smell, vision, taste, and hearing. Pain and temperature signals travel in the spinothalamic tract. These fibers cross 1 to 2 spinal nerve segments above where they enter, then move through the brainstem to the thalamus. From there, the...

neck exam

Cervical Spine and Neck Injury

The neck contains the cervical spine, a group of seven vertebrae arranged in a gentle C-shaped curve. These bones, joints, and ligaments make the neck complex to examine. The cervical spine supports the head and helps protect the nerves that come from the brain and spinal cord. Each vertebra has a thick bony body at the front. Intervertebral discs connect the bodies above and below, and they add stability and shock absorption. The back part of each vertebra includes the laminae, transverse and...

shoulder exam i

Shoulder Exam: Joint Structure and Motion

Shoulder exam starts with understanding the joints and muscles that control shoulder movement. The shoulder is made up of four separate joints: the glenohumeral (GH) joint, the acromioclavicular (AC) joint, the sternoclavicular joint, and the scapulothoracic joint. The GH joint is the main joint for shoulder motion. It is also the most mobile joint in the body. Because it is shaped like a golf ball sitting on a tee, it can be prone to instability. The GH joint is held in place by the four...

shoulder exam ii

Shoulder Strength and Instability Tests

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elbow exam

Elbow Injury Checks and Stability Tests

The elbow is a hinge joint made by the humerus, radius, and ulna. It is more stable than the shoulder, so it has less range of motion. The elbow and nearby structures can still be injured, especially with repetitive motion. Lateral epicondylitis and medial epicondylitis are two common elbow problems. Lateral epicondylitis is also called tennis elbow, and medial epicondylitis is also called golfer's elbow. These conditions often develop from occupational activities. A full elbow exam starts...

wrist and hand examination

Wrist and Hand Injury Exam

The wrist and hand injury exam checks bones, joints, tendons, nerves, and blood flow. The wrist is a complex joint made of 8 carpal bones and many ligaments. The hand has 5 metacarpal bones, and the fingers have 14 phalanges. Tendons and muscles lie over these bones and help move the hand and fingers. Wrist and hand problems can follow impact, weight-bearing activities such as gymnastics, or twisting and throwing motions. The exam starts with inspection. The examiner then looks for tenderness...

lower back exam

Lower Back Pain Evaluation

The lower back exam checks for pain, injury, and changes in the spine and nearby tissues. Back pain is a common source of pain in the body. Because the lower back includes bones, discs, ligaments, nerves, and muscles, each of these structures can contribute to symptoms. The exam starts with inspection and palpation. Inspection means looking for signs of deformity or inflammation. Palpation means feeling the area with the hands to check for tenderness or swelling. The exam also includes range...

hip exam

Assessing Hip Pain and Movement

The hip is a ball-and-socket joint made by the femoral head fitting into the acetabulum. The hip ligaments help make it a very strong and stable joint. Even so, the hip still has a wide range of motion and can wear down over time or after injury. Hip pain can affect people of many ages. It may be linked to problems inside the joint or outside the joint. The location of pain in the hip region can give early clues about the cause. A hip exam looks for asymmetry, swelling, and changes in gait,...

Knee injury assessment focuses on the largest joint in the body, where the femur meets the tibia. Because the knee sits in the middle of the lower leg, it faces traumatic and degenerative forces. Its structure also makes the exam more detailed. The joint is inherently unstable and relies on ligaments and menisci for support. The menisci act as shock absorbers and help increase the contact area of the joint. The patella sits in front of the knee and acts as a fulcrum for forceful extension.

ankle exam

How to Assess an Injured Ankle

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foot exam

Assessing Foot Pain and Injury

Foot pain and injury can be evaluated by checking the foot carefully from side to side. The foot is a complex structure made of many bones and joints. It gives the body flexibility, serves as the main point of contact for walking, and helps absorb shock. Because the foot supports the weight of the whole body, it is often prone to pain and injury. A full exam starts by removing shoes and socks on both feet. This lets the examiner inspect the entire foot and compare the painful or injured side...

physical examinations iii

This collection covers physical examination of two major systems in our body: neurological and musculoskeletal, with videos explaining relevant anatomy, the rationale behind the steps, and the interpretation of the exam findings.

Learn how the knee is examined for injury, from inspection and range of motion to ligament, meniscus, and patella tests used in high school anatomy.

Knee Injury Assessment | JoVE K12

Robert E. Sallis

Kaiser Permanente

Overview

Inspection and Palpation

Range of Motion Testing

Ligament Testing

Meniscal Tests

Patella Tests

Summary

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Visualizing Onion and Cheek Cells
ExpandImmediately before the experiment, wash and peel onion bulbs for the class.
Remove the entire brown outer skin and ...

Visualizing Onion and Human Cells
ExpandIn this experiment you will prepare different types of cells from a plant and animal, onion and human ...

What are Cells?

Cell Diversity

The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term &#34;cell&#34; based on the ...

Cytoplasm

The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The ...

The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression ...

The Central Dogma

The DNA Helix

Nucleic Acids

DNA as a Genetic Template

Structure of a Gene

A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the ...

Chromosome Structure

Base-pairing and DNA Repair

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised&#160; of nucleotides and proteins are ...

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form ...

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse ...

Transcription

Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. ...

Translation

Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes ...

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that ...

Protein and Protein Structures

Proteins: From Genes to Degradation

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of ...

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to ...

An Overview of Gene Expression

Gene expression is the complex process where a cell uses its genetic information to make functional products. This process is regulated at multiple ...

an-introduction-to-caenorhabditis-elegans

An Introduction to Caenorhabditis elegans

Caenorhabditis elegans is a microscopic, soil-dwelling roundworm that has been powerfully used as a model organism since the early 1970&rsquo;s.  It was ...

An Overview of Genetic Analysis

An organism&#8217;s physical traits, or phenotype, are a product of its genotype, which is the combination of alleles (gene variants) inherited from its ...

An Introduction to Cell Death

Necrosis, apoptosis, and autophagic cell death are all manners in which cells can die, and these mechanisms can be induced by different stimuli, such as ...

An Overview of Genetic Engineering

Genetic engineering &#8211; the process of purposefully altering an organism&#8217;s DNA &#8211; has been used to create powerful research tools and model ...

An Introduction to Cell Division

Cell division is the process by which a parent cell divides and gives rise to two or more daughter cells. It is a means of reproduction for single-cell ...

An Overview of Genetics and Disease

Many human diseases are associated with mutations or variations in genetic sequences. Some of these genetic variants are heritable, passed down from ...

an-introduction-to-cellular-and-molecular-neuroscience

An Introduction to Cellular and Molecular Neuroscience

Cellular and molecular neuroscience is one of the newest and fastest growing subdisciplines in neuroscience. By investigating the influences of genes, ...

Annexin V and Propidium Iodide Labeling

Staining with annexin V and propidium iodide (PI) provides researchers with a way to identify different types of cell death&#8212;either necrosis or ...

an-introduction-to-developmental-genetics

An Introduction to Developmental Genetics

Development is the complex process through which a single-celled embryo transforms into a multicellular organism. Developmental processes are guided by ...

Bacterial Transformation: Electroporation

The term &#x201C;transformation&#x201D; refers cellular ingestion of foreign DNA.  In nature, transformation can occur in certain types of bacteria.  In ...

an-introduction-to-molecular-developmental-biology

An Introduction to Molecular Developmental Biology

Molecular signals play a major role in the complex processes occurring during embryonic development. These signals regulate activities such as cell ...

bacterial-transformation-the-heat-shock-method

Bacterial Transformation: The Heat Shock Method

Transformation is the process that occurs when a cell ingests foreign DNA from its surroundings. Transformation can occur in nature in certain types of ...

an-introduction-to-saccharomyces-cerevisiae

An Introduction to Saccharomyces cerevisiae

Saccharomyces cerevisiae (commonly known as baker&rsquo;s yeast) is a single-celled eukaryote that is frequently used in scientific research.  S. ...

Cell Cycle Analysis

Cell cycle refers to the set of events through which a cell grows, replicates its genome, and ultimately divides into two daughter cells through the ...

An Introduction to Transfection

Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell ...

Chromatin Immunoprecipitation

Histones are proteins that help organize DNA in eukaryotic nuclei by serving as &#8220;scaffolds&#8221; around which DNA can be wrapped, forming a complex ...

An Overview of Epigenetics

Since the early days of genetics research, scientists have noted certain heritable phenotypic differences that are not due to differences in the ...

community-dna-extraction-from-bacterial-colonies

Community DNA Extraction from Bacterial Colonies

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona
Demonstrating Author: Luisa Ikner
Traditional methods of analysis ...

Cytogenetics

Cytogenetics is the field of study devoted to chromosomes, and involves the direct observation of a cell&#8217;s chromosomal number and structure, ...

embryonic-stem-cell-culture-and-differentiation

Embryonic Stem Cell Culture and Differentiation

Culturing embryonic stem (ES) cells requires conditions that maintain these cells in an undifferentiated state to preserve their capacity for self-renewal ...

DNA Gel Electrophoresis

DNA gel electrophoresis is a technique used for the detection and separation of DNA molecules. An electric field is applied to a gel matrix comprised of ...

Enzyme Assays and Kinetics

Enzyme kinetics describes the catalytic effects of enzymes, which are biomolecules that facilitate chemical reactions necessary for living organisms. ...

DNA Ligation Reactions

In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond.  An enzyme known as a ligase ...

explant-culture-for-developmental-studies

Explant Culture for Developmental Studies

Explant culture is a technique in which living cells or tissues are removed from an embryo for continued development outside of the organism. This ex vivo ...

DNA Methylation Analysis

Methylation at CpG dinucleotides is a chemical modification of DNA hypothesized to play important roles in regulating gene expression. In particular, the ...

Expression Profiling with Microarrays

Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and ...

Density Gradient Ultracentrifugation

Density gradient ultracentrifugation is a common technique used to isolate and purify biomolecules and cell structures. This technique exploits the fact ...

F&#246;rster Resonance Energy Transfer (FRET)

F&#246;rster resonance energy transfer (FRET) is a phenomenon used to investigate close-range biochemical interactions. In FRET, a donor photoluminescent ...

detecting-environmental-microorganisms-with-polymerase-chain-reaction

Detecting Environmental Microorganisms with the Polymerase Chain Reaction and Gel Electrophoresis

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - Arizona University
Demonstrating Author: Bradley Schmitz
Polymerase chain reaction (PCR) is ...

Gel Purification

Gel purification is used to recover DNA fragments after electrophoretic separation. DNA recovery from an agarose gel includes three basic steps: binding, ...

Detecting Reactive Oxygen Species

Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are ...

Gene Silencing with Morpholinos

Morpholino-mediated gene silencing is a common technique used to study roles of specific genes during development. Morpholinos inhibit gene expression by ...

development-and-reproduction-of-the-laboratory-mouse

Development and Reproduction of the Laboratory Mouse

Successful breeding of the laboratory mouse (Mus musculus) is critical to the establishment and maintenance of a productive animal colony. Additionally, ...

Genetic Crosses

To dissect genetic processes or create organisms with novel suites of traits, scientists can perform genetic crosses, or the purposeful mating of two ...

drosophila-melanogaster-embryo-and-larva-harvesting-and-preparation

Drosophila melanogaster Embryo and Larva Harvesting and Preparation

Drosophila melanogaster embryos and larvae are easy to manipulate and develop rapidly by mechanisms that are analogous to other organisms, including ...

Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins which carry out the essential functions of life through systems of specialized cells.

HS-LS1-1.

Knee Injury Assessment

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