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Basic Methods in Cellular and Molecular Biology
Plasmid DNA Cleanup from Bacterial Cells
Description
Plasmid DNA cleanup from bacterial cells is a lab method for separating plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA molecule that carries a specific DNA fragment. After transformation into a host organism, the plasmid is replicated and ma...
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Transcript
Plasmids are circular, extra chromosomal, DNA molecules and in molecular biology they act as carriers, or vectors, for a specific DNA fragment. Bacteria are used to replicate plasmids, so that your DNA of interest is mass-produced. The process by which researchers obtain plasmids from bacteria is called plasmid purification, ...
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