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Basic Methods in Cellular and Molecular Biology
Plasmid Purification
Description
Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA that is used as a carrier of specific DNA molecules. When introduced into a host organism via transformation, a plasmid will be replicated, creating nu...
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Transcript
Plasmids are circular, extra chromosomal, DNA molecules and in molecular biology they act as carriers, or vectors, for a specific DNA fragment. Bacteria are used to replicate plasmids, so that your DNA of interest is mass-produced. The process by which researchers obtain plasmids from bacteria is called plasmid purification, ...
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