Tracheotomy: A Method for Transplantation of Stem Cells to the Lung

Summary

Significant breakthroughs in stem cell identification are continuously being made. To translate these discoveries, however, novel methods for cellular delivery must be devised. Here I report that the airways provide a safe route for stem cell transplantation to the lungs.

Abstract

Lung disease is a leading cause of death and likely to become an epidemic given increases in pollution and smoking worldwide. Advances in stem cell therapy may alleviate many of the symptoms associated with lung disease and induce alveolar repair in adults. Concurrent with the ongoing search for stem cells applicable for human treatment, precise delivery and homing (to the site of disease) must be reassured for successful therapy. Here, I report that stem cells can safely be instilled via the trachea opening a non-stop route to the lung. This method involves a skin incision, caudal insertion of a cannula into and along the tracheal lumen, and injection of a stem cell vehicle mixture into airways of the lung. A broad range of media solutions and stabilizers can be instilled via tracheotomy, resulting in the ability to deliver a wider range of cell types. With alveolar epithelium confining these cells to the lumen, lung expansion and negative pressure during inhalation may also assist in stem cell integration. Tracheal delivery of stem cells, with a quick uptake and the ability to handle a large range of treatments, could accelerate the development of cell-based therapies, opening new avenues for treatment of lung disease.

Protocol

Pre-operative

1. Recipient mouse and donor cells
2. Operating platform with tape and suture thread
3. 1cc syringe
4. 23G needle
5. Anesthetic: avertin 25 mg/ml (alternatively ketamine 9 mg/ml and xylazin 1 mg/ml)
6. Hair clipper with #40 blade
7. Sterile gloves
8. Alcohol swabs
9. Iodine
10. Sterilized surgical instruments
11. Suture thread
12. 22G catheter
13. 200 ul pipette (sterile)
14. Sterilized pipette tips
15. Antibiotic glue ointment
16. Sharpie container

Operative

Aseptic techniques ar....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
23 G needle
1 cc syringe
22 G catheter
suture thread
antibiotic glue ointment
Anesthetic			Avertin 25 mg/mL. Alternatively ketamine 9 mg/mL and xylazin 1 mg/ml.