The umbilical cords are used to isolate smooth muscle cells by different ways. In this work we used the enzymatic treatment to isolated smooth muscle cells.
Cleanup
The SMC can be used as models for future assays with constrictor drugs, to isolate and structurally characterize L-type calcium channels, to study cellular and molecular aspects of the vascular function and to use them in tissue engineering. SMCs and ECs are fundamental for the development of new biomaterials that can be used in the production of semi synthetic blood vessels to be used in humans.
Hospital Amato Lusitano, Castelo Branco Portugal.
Hospital Sousa Martins, Guarda Portugal.
Material Name | Type | Company | Catalogue Number | Comment |
---|---|---|---|---|
DMEM-F12 | Sigma-Aldrich | 095K8311 | ||
Fetal bovine serum (FBS) | Biochrom-AG | 0117T | ||
RPMI 1640 | Gibco | 3103806 | ||
Collagen | Sigma-Aldrich | 067K2302 | ||
Antibiotic | 10 μL/ 1 mL of medium | |||
Scissors | Sterilized | |||
Needle | Diameter of 0.6-1mm | |||
Syringe | 10 – 20 mL | |||
Physiological Saline Solution (PSS) | 20-40 mL per artery | |||
Collagenase type I | Sigma-Aldrich | 047K1052 | 3 mL per artery | |
Hank’s Buffered Salt Solution(HBSS) | Diluted collagenase | |||
Phosphate buffered saline solution (PBS) | Warmed to 37°C |