Fetal Bovine Serum
Fetal Bovine Serum - Related Videos
07:51
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
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Cited by 44 •
2015
Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
08:26
Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)
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2018
The protocols for studying the binding of gold cations (Au(III)) to various conformations of bovine serum albumin (BSA) as well as for characterizing the conformational dependent unique BSA-Au fluorescence are presented.
13:37
Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture
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Cited by 34 •
2012
This protocol illustrates a harvesting technique for coccygeal bovine intervertebral discs for organ culture for in vitro organ culture.
09:35
An Experimental System to Study Mechanotransduction in Fetal Lung Cells
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Cited by 7 •
2012
Mechanical forces play a key role in lung development and lung injury. Here, we describe a method to isolate rodent fetal lung type II epithelial cells and fibroblasts and to expose them to mechanical stimulation using an in vitro system.
08:04
Murine Fetal Echocardiography
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Cited by 7 •
2013
Fetal and perinatal death is a common feature when studying genetic alterations affecting cardiac development. High-frequency ultrasound imaging has improved 2-D resolution and can provide excellent information on early cardiac development and is an ideal method to detect the impact on cardiac structure and function prior to death.
FAQs
Its proteins, lipids, hormones, and growth factors contribute different forms of nutritional and regulatory support in culture. These components can promote cell attachment and proliferation while helping cells tolerate stresses associated with growth outside the body. Their combined activity makes the serum useful when cells require a complex environment rather than a single defined supplement.
FBS composition can vary between batches, so cells may receive different concentrations or combinations of biologically active components. Such variation can influence how well cells attach, proliferate, or respond to culture-related stress. Researchers therefore need to consider batch-related differences when maintaining cultures or comparing results across experiments.
Serum-free and defined alternatives are important because FBS has variable composition and raises ethical and scientific concerns related to its collection and use. A defined formulation can help researchers evaluate specific culture components more directly. These alternatives are especially relevant when reproducibility, controlled conditions, or reduced dependence on animal-derived material matters.
Researchers add Fetal Bovine Serum to culture media, where it supplies a mixture of proteins, lipids, hormones, growth factors, and other components. The supplemented medium supports cell attachment, proliferation, and protection from culture-related stress. This approach enables the maintenance and expansion of both primary cells and established cell lines outside the body.
FBS-supported cultures can provide experimental systems for studying cell physiology, disease, and drug responses. They also support tissue-engineering work by helping maintain and expand cells under laboratory conditions. Because both primary cells and established cell lines can be cultured with this support, researchers can investigate biological processes across several experimental models.
Selection requires attention to the serum’s variable composition, its role in supporting the intended cells, and the scientific or ethical concerns associated with collection. Researchers should also consider whether serum-free or defined alternatives can meet the study’s needs. These factors affect culture consistency and the interpretation of biological outcomes.