Chronic Salmonella Infected Mouse Model

Summary

Establish a chronic bacterial infected mouse model with persistent Salmonella typhimurium colonization in intestine for 27 weeks.

Abstract

The bacterial infected mouse model is a powerful model system for studying areas such as infection, inflammation, immunology, signal transduction, and tumorigenesis. Many researchers have taken advantage of the colitis induced by Salmonella typhimurium for the studies on the early phase of inflammation and infection. However, only few reports are on the chronic infection in vivo. Mice with Salmonella persistent existence in the gastrointestinal tract allow us to explore the long-term host-bacterial interaction, signal transduction, and tumorigenesis. We have established a chronic bacterial infected mouse model with Salmonella typhimurium colonization in the mouse intestine over 6 months. To use this system, it is necessary for the researcher to learn how to prepare the bacterial culture and gavage the animals. We detail a methodology for prepare bacterial culture and gavage mice. We also show how to detect the Salmonella persistence in the gastrointestinal tract. Overall, this protocol will aid researchers using the bacterial infected mouse model to address fundamentally important biological and microbiological questions.

Protocol

This protocol includes three portions: bacterial culture, mouse gavage, and Salmonella detection.

1. Salmonella growth condition.

1. Prepare the Salmonella Luria-Bertani broth (LB) plate, incubate at 37° C overnight.
2. Pick a clone from the LB plate and put into 7 ml LB in a 12 ml tube, and shake at 37°C for about 5 hours.
3. Inoculate 50 ml LB with 0.05 ml of a stationary phase culture and incubate at 37°C without shaking for about 18 hours.
4. Spin overnight bacterial culture at room temperature with 6000 rpm for 10 minutes, suspend the bacteria in HBSS using ratio 100:3 (LB:....

Discussion

To use this system, it is necessary for the researcher to learn how to gavage the animals. We detail a methodology for prepare bacterial culture and gavage the mice. We also show how to monitor the Salmonella persistence in the gastrointestinal (GI) tract. The critical steps in this protocol including:

1. Streptomycin-pretreatment: Streptomycin-pretreatment could get rid of some commensal gut flora and make the mice susceptible to the Salmonella infection².
2. Salmonell.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
HBSS	Sigma-Aldrich	ABCD1234
Luria-Bertani broth	BD Biosciences	244610
Feeding needle	Popper & Sons, Inc.	7920
Streptomycin	MP Biomedicals	100556
BBL CHROMagar Salmonella	BD Biosciences	214983
Disposable cell spreaders	Biologix Research Company	65-1010