Larval Locomotion Assay: High-Throughput Method to Study Neurobehavioral Toxicity of a Compound in Zebrafish Larvae

Abstract

Source: Zhang B., et al. Studying Neurobehavioral Effects of Environmental Pollutants on Zebrafish Larvae. J. Vis. Exp. (2020)

This article describes zebrafish’s behavioral response to toxic compounds by monitoring larval locomotion. The protocol further looks at an example of zebrafish larvae’s response to environmental pollutant.

Protocol

1. Preparation for the behavior test

1. Transfer 800 µL of exposure solution into every well of the 48 well microplate.
   NOTE: Use 16 wells for every group (i.e., the control solution, 5 µg/L, and 50 µg/L group).
2. Use a 1 mL pipette tip to transfer 200 µL of exposure solution with one larva from the glass Petri dish into one well of the 48 well microplate.
3. Transfer 4 mL of exposure solution into every well of the 6 well microplate.
   NOTE: Use one 6 well microplate for every group.
4. Use a 1 mL pipette tip to transfer 200 µL of exposure solution w....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
48-well-microplate	Corning	3548	Embyros housing
6-well-microplate	Corning	3471	Embyros housing
Zebrabox	Viewpoint	ZebraBox	Behavior instrument
ZebraLab	Viewpoint	ZebraLab	Behavior software
Pipette	Eppendorf	3120000267	Transfer solution