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Method Article
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April 30th, 2023
Source: Katsuta, E., et al. Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy. J. Vis. Exp (2018).
This video describes a technique of radical mastectomy, which is used for the surgical removal of the entire mammary gland from a mouse to study cancer progression.
1. Mastectomy
NOTE: The timing of the mastectomy is very important. If it is done too early, lung metastasis does not occur. If it is done too late, the primary tumor has invaded major blood vessels, which make a complete oncologic resection challenging. Thus, multiple time points were tested for mastectomy to determine which time point produced the appropriate balance in waiting for metastasis before resection became too challenging. After doing so in over 50 mouse experiments, it was demonstrated that mastectomy at 8 days after cancer cell inoculation (or when the tumor size reaches 5 mm) was the ideal time point to ac....
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| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| Micro Dissection Scissors | Roboz | RS-5983 | For cancer cell inoculation and mastectomy |
| Adson Forceps | Roboz | RS-5233 | For cancer cell inoculation and mastectomy |
| Needle Holder | Roboz | RS-7830 | For cancer cell inoculation and mastectomy |
| 5-0 silk sutures | Look | 774B | For cancer cell inoculation and mastectomy |
| Dry sterilant (Germinator 500) | Braintree Scientific | GER 5287-120V | For cancer cell inoculation and mastectomy |
| Clipper | Wahl | 9908-717 | For cancer cell inoculation and mastectomy |
