Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo

Abstract

Source: Moreno, J. A., et al. Fluorescent orthotopic mouse model of pancreatic cancer. J. Vis. Exp.(2016)

This video demonstrates the protocol for implanting green fluorescent protein-expressing pancreatic cancer cells orthotopically into the pancreas of the mouse. The orthotopic mouse model helps in investigating the efficacy of novel therapeutics on primary and metastatic tumors.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the jove veterinary review board.

1. Surgical Implantation

NOTE: Ensure that all surgical materials and instruments are sterile. Practice aseptic techniques at all times.

1. Place a heating pad on the table and cover with a sterile drape. Set up the anesthesia machine and ensure all the supplies are within arm's reach.
2. Place the animal's snout into the anesthesia mask and set up the anesthesia to 1 L/min of oxygen and 2.5% isoflurane.

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Alcare or Foamed Antiseptic Handrub	Steris	639680
TB Syringe 27 G 1/2	Becton Dickinson	305620
Isoflurane	Blutler Schein	50562
Ketoprofen	Fort Dodge Animal Health
Surgical Scissors, 5.5" straight mayo	Henry Schein	22-1600
PANC-1 GFP cell line	Anticancer, Inc
Small Animal Imaging System: iBox Scientia	UVP, LLC. Upland, CA.		Small Animal Imaging System to observe the fluorescent tumor in live animals
SutureVet PGA 6-0 PGA	Henry Schein	39010
PANC-1 GFP cell line	Anticancer, Inc
DPBS (Dubelcco's Phosphate Buffered Saline)	Thermo Ficher Sci	21300025