Intracisternal Delivery of Meningococci into Mouse Models to Induce Meningococcal Meningitis

Abstract

Source: Pagliuca, C., et al., Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery. J. Vis. Exp. (2019)

In this video, we demonstrate the intracisternal delivery of Neisseria meningitidis into a mouse model to generate meningitis infection.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Intra-cisternal injection of mice

NOTE: The entire procedure is performed in the laminar flow cabinet to maintain aseptic conditions.

1. House laboratory mice (8-week-old, female Balb/c) under specific pathogen-free conditions. Provide food pellets and water ad libitum.
2. Settle the animals in the new environment for 1 week before starting the experiment.
3. Before starting the experiment, weigh and ev....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Alarm-Thermometer	TESTO	9000530	Device
BD Micro Fine syringe	BD	320837	U-100 Insulin
BD Plastipak syringe 1ml 25GA 5/8in	BD	300014	05x16mm
BD Plastipak syringe 5ml	BD	308062	07 x 30mm
BIOHAZARD AURA B VERTICAL LAMINAR FLOW CABINET	Bio Air s.c.r.l.	Aura B3	Device
C150 CO2 Incubator	Binder	9040-0078	Device
Diete Standard Certificate	Mucedola s.r.l.	4RF21	Food pellet for animal
Dumont Hp Tweezers 5 Stainless Steel	F.S.T. by DUMONT	AGT5034	0,10 x 0,06 mm tip
Electronic Balance	Gibertini	EU-C1200	Max 1200g, d=0.01g, T=-1200g
Iron dextran solution	Sigma-Aldrich	D8517-25ML
Ketamine	Intervet
Microbiological Safety Cabinet BH-EN and BHG Class II	Faster	BH-EN 2004
Microcentrifuge tubes 1.5ml	BRAND	PP780751	Screw cap PP, grad
Mouse Handling Forceps	F.S.T.	11035-20	Serrated rubber; Gripping surface:15 x 20 mm
Natural Latex Gloves	Medica	M101
Touch N Tuff disposible nitrile gloves	Ansell	92-500
Xylazine	Intervet