Implantation of Electrodes into a Mouse Cortex for Electrocorticographic Recordings

In This Article

Abstract

Source: Dufort-Gervais, et al. Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice. J. Vis. Exp. (2021)

This video demonstrates a protocol for implanting electrocorticographic electrodes in the mouse cerebral cortex to record electrical signals in response to adeno-associated viruses that target and manipulate cofilin expression.

Protocol

1. ECoG/EMG electrode implantation

Using straight Kelly forceps, slowly screw one ECoG electrode (with straight gold wire) in the vertical axis in the hole where the AAV was injected. Leave at least 2.5 mm of the screw out of the skull to minimize damage to the dura and cerebral cortex (i.e., for an approximate depth of 1.1 mm from the skull surface; Figure 1D).
Mark the position of the posterior ECoG electrode and the reference electrode on the skull with a pen at these coordinates: posterior electrode (visual cortex) 1.5 mm lateral right to midline and 1.5 mm anterior to lambda, reference electrode (somatosenso....

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Results

Neural implant procedure on rat; setup includes electrodes, connectors, and fixation apparatus.
Figure 1: Preparation of ECoG/EMG montage components and representative example of ECoG electrode placement. (A) An ECoG electrode: a 4 mm long, 0.2 mm diameter gold wire (non-insulated) is fused on the head of a gold-covered screw (1.9 mm h.......