Visualization and Mapping of Methoxy-X04-Labeled Amyloid Plaques in an Alzheimer’s Disease Mouse Brain Section

Abstract

Source: Bisht, K., et. al. Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques. J. Vis. Exp. (2016)

This video demonstrates the visualization and mapping of Methoxy-X04-labeled amyloid plaques in Alzheimer’s disease mouse brain sections using fluorescence and bright-field microscopy, enabling precise correlation of plaques with anatomical structures for studying Alzheimer’s pathology.

Protocol

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Methoxy-X04	Tocris Bioscience	4920	10 mg substrate per tablet
Microscope Slides	Fisher Scientific	12-550-15
24-well Tissue Culture Plates	Fisher Scientific	353047
Ethylene Glycol	Fisher BioReagents	BP230-4
Glycerol	Fisher BioReagents	BP229-4