Visualization of Mitochondria within Intraepidermal Nerve Fibers

Abstract

Source: Hamid, H. S., et al. Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers. J. Vis. Exp. (2017)

This video demonstrates the method for visualizing nerve-specific mitochondria using three-dimensional imaging. It outlines the process of acquiring sequential scans of pre-labeled intraepidermal nerve fibers and generating 3D surface images to isolate and display mitochondria in relation to nerve structures.

Protocol

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Leica SP5 laser scanning confocal microscope	Leica Microsystems, Buffalo Grove, IL	SP5
Volocity x64 software	Perkin Elmer, Waltham , MA	Version 4.4.0	Volocity software is used for image processing
Imaris x64 3D analysis software	Bitplane, Concord, MA	Version 7.7.1	Imaris software is used for image analysis