In this video, human-induced pluripotent stem cell-derived neural cells are stereotaxically injected into the gray matter-white matter border of the deep cortex in a mouse model of traumatic brain injury. The transplanted cells integrate into the tissue, promoting functional recovery and circuit repair in the injured brain.
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Method Article
Stereotaxic Transplantation of Neural Cell Suspension into a Mouse with Traumatic Brain Injury
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August 29th, 2025
In This Article
Abstract
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Stereotaxic transplantation of cell suspension
- Begin cell transplantation procedure roughly 24 h after craniectomy.
- Prepare the cell transplantation equipment and surgical supplies
- Fill a 1 mL slip-tip syringe with sterile saline for wound irrigation. Attach a 25 G needle to the syringe to control irrigation.
- Fill a 1 mL slip-tip syringe with CsA (Cyclosporine A) solution for immunosuppression. Attach a 25 G needle or larger to the CsA syringe. Refill the CsA syringe as n....
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Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 1 ml syringes | Becton Dickinson (BD) | 309659 | |
| 1.7 ml flip top test tubes | Denville | C2170 | |
| 10 microliter syringe | Hamilton | 7635-01 | |
| 25G Precision Glide syringe needles | Becton Dickinson (BD) | 305122 | |
| 70% Ethanol | Product of choice; varies by region | ||
| Acetaminophen oral suspension | Tylenol (Children's) | Dilute to 1 mg/ml in water | |
| Anesthetic vaporizer | Vetland | 0521-11-22 | |
| Animal handling cloth | Purchase from department store | ||
| Betadine | Purdue Products | NDC-67618-151-32 | |
| Compressed oxygen | Product of choice; varies by region | ||
| Cyclosporine A | Sigma-Aldrich | 30024-100mg | |
| Dimethyl sulfoxide (DMSO) | Sigma-Aldrich | D8418-500ml | |
| DMEM | Invitrogen (ThermoFisher) | A14430-01 | |
| Electrical tape | 3M Corporation | Purchase from department store | |
| Fine tweezers | Fine Science Tools | 11254-20 | |
| Forceps | Fine Science Tools | 91106-12 | |
| Glass capillary pipettes, 1 mm OD, 0.58 mm ID | World Precision Instruments | 1B100F-3 | |
| High Speed Rotary Micromotor Kit | Foredom Electric Co. | K.1070 - K.107018 | |
| Ideal Micro Drill Burr Set Of 5 | Cell Point Scientific | 60-1000 | |
| Impact One Stereotaxic Impactor for CCI | Leica Biosystems | 39463920 | |
| Isoflurane | Baxter | NDC-10019-360-60 | |
| Lab bench timers | Fisher Scientific | 14-649-17 | |
| Micropipette puller | MicroData Instruments, Inc. | PMP-102 | Any puller will suffice |
| Microscope cover slips | Fisherbrand | 12-545-E | |
| Microscope slide mounting medium | Product of choice | ||
| Mirror | Purchase from department store | ||
| Needle holder hemostat | Fine Science Tools | 12002-12 | |
| Ophthalmic ointment | Falcon Pharmaceuticals | NDC-61314-631-36 | |
| Ophthalmic spring scissors | Fine Science Tools | 15018-10 | |
| Plastic box | Purchase from department store | ||
| Plastic cylinder | Purchase from department store | ||
| QSI motorized syringe pump | Stoelting | 53311 | |
| Removable needle compression fitting | Hamilton | 55750-01 | |
| Small rodent stereotaxic frame | Stoelting | 51925 | |
| Small scissors | Fine Science Tools | 14060-09 | |
| StemPro Accutase | Invitrogen (ThermoFisher) | A1110501 | |
| Sterile alcohol prep pads | Fisherbrand | 06-669-62 | |
| Sterile cotton swabs/Kendall Q-tips | Tyco Healthcare | 540500 | |
| Sterile saline | Hospira | NDC-0409-1966-07 | |
| Stopwatches (2) | Fisher Scientific | 06-662-56 | |
| Superfrost Plus Gold microscope slides | Fisherbrand | 15-188-48 | |
| Sutures - 5.0 silk with curved needle | Oasis | MV-682 |
