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Method Article

Wireless Surface Electromyography Recording of Facial Muscle Activity During Expressions

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November 10th, 2025

In This Article

Abstract

Source:
Hila Man1, Paul F. Funk2, Ieva Vebraite-Adereth3, Christoph Anders4, Orlando Guntinas-Lichius2, Yael Hanein1,3,5

1Sagol School of Neuroscience, Tel Aviv University, 2Department of Otorhinolaryngology, Jena University Hospital, Friedrich-Schiller-University Jena, 3School of Electrical Engineering, Tel Aviv University, 4Division Motor Research, Pathophysiology and Biomechanics, Department of Trauma, Hand and Reconstructive Surgery, Jena University Hospital, Friedrich-Schiller-University Jena, 5X-trodes Ltd.

This video demonstrates the procedure for non-invasive recording of facial muscle activity using wireless surface electromyography (sEMG) during video-guided facial expressions performed by a participant.

Protocol

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board.

1. Preparation of equipment and software

  1. Charge data acquisition unit (DAU): Charge the wireless DAU fully before the experiment.
  2. Prepare a secure digital (SD) card: Insert a formatted v30-type SD card inside the DAU and turn it ON.
  3. Minimize disruptions: Place all cellular phones in quiet mode and turn off Bluetooth to avoid interference.
  4. Tablet setup
    1. Install and open the e....

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Results

Facial muscle electromyography diagram; electrode placement, calibration, experiment flowchart.

Figure 1: Schematic experimental setup overview. (A) Positions of the electrodes on the face: 1-16 .......

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Materials

List of materials used in this article
NameCompanyCatalog NumberComments
Alcohol prep padMEDI PLUS7290010993833To clean the face area for electrode placement.
Android tabletSamsungGalaxy Tab S7To run electrode application
DAUX-trodes Ltd.XTR-BT V1.3Wireless data acquisition unit
Facial expression experiment fileN/AN/Ahttps://github.com/NeuroEngLabTAU/facial_expressions_experiment
Long Type-C to Type-C cableN/AN/AConnection of the Tablet to the PC
Micro-SD cardSanDiskN/ATo place into DAU and store recorded data
NuPrep skin prep gelWeaver and companyhttps://www.weaverandcompany.com/products/nuprep/ To clean the face area for electrode placement. It lowers the skin impedance with minimal skin irritation and patient discomfort.
PC monitorPhillips, Dell For viewing experiment instructions
Personal computer (PC)AntecN/AWindows 10+; Processors: Intel i7 processor. BT receiver.
USB SD card readerSIYOTEAMSY-368To transfer recorded EMG data from SD card to the PC
XTR EXG16X-trodes Ltd.XTELC0004005RMDisposable 16-channel wearable dry electrode array patch for EMG, ECG monitoring
X-trodes applicationX-trodes Ltd.N/AAn application for recording data from X-trodes electrodes on Android tablet

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