Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.
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Method Article
Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region
In This Article
Summary
Abstract
Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.
Protocol
Step 1
Sacrifice the rat according to established protocols. Remove the brain from the skull and rinse it in ice cold DEPC treated Milli Q water to remove any surface blood.
Step 2
Place on cold metal plate. Cut the brain bi-half into right and left hemisphere.
Figure ....
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Discussion
Different brain regions associate with different neurodegenerative diseases. For example, frontal cortex and striatum relate with Parkinson s disease while hippocampus relates with Alzheimer's disease. Precise micro-dissection of the brain allows us to detect the mRNA and protein changes in diseased region more accurately.
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Disclosures
The animals were handled according to the protocol for the use of animal in research approved by Committee on the Use of Live Animals in Teaching and Research (CULATR) in the University of Hong Kong and according to the National Institutes of Health guidelines for animal use.
Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| Microscope | Olympus Corporation | ||
| Dissection tools | Metal plate, forceps, blade, etc. Individually wrapped with aluminum foil and heated at 180oC for eight hours to eliminate RNase. Made ice cold before use. |
References
- Palkovits, M. Maps and guide to micro-dissection of the rat brain. , Elsevier. New York. (c.1988).
- Swanson, L. W. Brain maps: structure of the rat brain. 3rd revised edition. , Elsevier Academic Press. (2004).
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