Renal Capsule Tumor Xenografting: A Technique to Generate an Experimental Animal Model to Evaluate Prostate Carcinogenesis

Abstract

Source: Nicholson, T. M. et al. Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia. J. Vis. Exp. (2013)

In this video, we demonstrate the implantation of human-derived prostate cancer tissue grafts into the renal capsule of an immunocompromised recipient mouse. This technique can be used to assess tumor progression and develop novel therapeutic strategies.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Surgical Procedure: Renal Capsule Xenografting

1. Prior to induction of anesthesia, observe the mouse to ensure health and wellbeing.
2. Place the mouse into the chamber for induction of isoflurane anesthesia at 3-5%. When the animal has stopped moving and the respiratory rate has decreased, transfer to the nose cone in the prone position, and maintain anesthesia at 1-3%.
3. If necessary, use clippers to shave the back of the mouse. This is not needed when....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Sterile surgical gown	Midwest Vet Supply	350.79866.2
Surgical mask	Midwest Vet Supply	350.50111.2
Sterile Gloves	Kimberly Clark	55092
Reflex Wound Clipper	F.S.T.	12031-09	Correspond w/ wound clip size
Vicryl Suture	Midwest Vet Supply	295-92100.2	4-0, FS-2, Absorbable
Straight Sharp/Blunt Scissors	Fine Scientific Tools (F.S.T.)	14054-13
Graefe forceps (Serrated, Toothed, Curved)	F.S.T.	11055-10
Isoflurane Vaporizer	Supera Anesthesia Innov	VAP3000
Induction Chamber	Supera Anesthesia Innov	RES644
Glass Pasteur pippets	Fisher Scientific	S67050	5" length
Graefe forceps (Serrated, Curved)	F.S.T.	11052-10
Graefe forceps (Serrated, Straight)	F.S.T.	11050-10
Betadine	Webster Veterinary	07-836-3379
Sterile saline	Midwest Vet Supply	193.74504.3	NaCl 0.9%, Injectable
Cotton Tip Applicator	Midwest Vet Supply	001.06220.2
Wound Clips	Braintree Scientific	ACS CS	May use 7mm or 9mm clips
Isoflurane	Midwest Vet Supply	193.33161.3
Dissecting microscope	Leica	L2
Small Mice Nose Cone	Supera Anesthesia Innov	ACC526
Oxygen Flowmeter	Supera Anesthesia Innov	OXY660
Would Clip Remover	F.S.T.	12033-00	Universal