Murine Vertical Sleeve Gastrectomy: A Surgical Procedure to Downsize Stomach in Experimental Mouse Models

Abstract

Source: Garibay, D., Cummings, B. P. A Murine Model of Vertical Sleeve Gastrectomy. J. Vis. Exp. (2017)

In this video, a vertical sleeve gastrectomy procedure is performed on an experimental mouse model. This procedure helps remove a significant portion of the stomach along its greater curvature.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Vertical Sleeve Gastrectomy and Sham Procedures

1. On the day of surgery, weigh mice to obtain a baseline weight measurement.
2. Equip the surgical room with an anesthesia system, heated water bath, and heating pad. Set the water bath to 37 °C and use it to warm 0.9% saline solution for irrigation. Clean the surgical field with 70% ethanol and use sterile technique to open autoclaved surgery pack and tools.
   NOTE: The heating pad is us....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
6-0 Suture	Ethicon	Z432	Monofilamentabsorbable/taper
7-0 Suture	Covidien	8866127-01	Monofilamentabsorbable/taper
Cotton swabs	Fisherbrand	23-400-118	Small
Cotton swabs	Fisherbrand	233-400-101	Large
Foil	Various
Surgery drape	Various
0.9% Saline solution	Various
Microneedle driver	Fine Science Tools	12075-14
Spring scissors	Fine Science Tools	15396-00
Alcohol	Various
Eye Ointment	Paralube®Vet Ointment	17033-211-38
Meloxicam (Metacam)	Boehringer Ingelheim	141-213	5 mg/ml
Thin tipped hemostats	Fine Science Tools	13021-12
20 mL syringe	Various
Enrofloxacin	Baytril	08713254-186599	22.7 mg/ml