All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. The UUO Surgery
<ol>
	<li>Prepare the following items: isoflurane, cork sheet, depilatory cream, laboratory wipes, Petri dish with PBS, 4-0 silk, 4-0 nylon, a 2.5 mL syringe, cotton swabs, tweezers, surgical scissors, and 8-week old C57BL/6 male mice.</li>
	<li>Anesthetize a mouse with 1.5% isoflurane and maintain at 1.5%. Then, apply depilatory cream to the mouse's abdomen. After a few minutes, wipe the depilatory cream off with a PBS-soaked laboratory wipe.</li>
	<li>Inject 70% ethanol into the mouse's abdomen and place the mouse in the supine position on the cork sheet.</li>
	<li>Using surgical scissors and tweezers, make an incision in the skin at the abdomen and cut the muscle and peritoneal membrane from the bladder to the left lower edge of the ribs.</li>
	<li>Place the 2.5 mL syringe underneath the mouse. Take two cotton swabs and moisten them with PBS. Pull the intestines carefully to the side with the tweezers and place the moistened swabs appropriately to identify the left ureter. Using the tweezers, lift the left kidney.</li>
	<li>Use the 4-0 silk to ligate the left ureter in two places approx. 1 cm apart. Cut the ureter at the center point of the two ligations and then use 4-0 nylon sutures to close the peritoneal membrane and incision.</li>
</ol>

unilateral ureteral obstruction murine model: a surgical procedure to obstruct the urinary flow from a kidney in mouse models

Unilateral Ureteral Obstruction Murine Model: A Surgical Procedure to Obstruct the Urinary Flow from a Kidney in Mouse Models

Source: Yanai, K. et al. <a href='https://www.jove.com/video/61383' target='_blank'>Quantitative real-time PCR evaluation of microRNA expressions in mouse kidney with unilateral ureteral obstruction.</a> J. Vis. Exp. (2020)
This video demonstrates the procedure to generate a unilateral ureteral obstruction mouse model. The procedure involves cutting one of the ureters of the mouse to obstruct urine flow.

Source: Yanai, K. et al. Quantitative real-time PCR evaluation of microRNA expressions in mouse kidney with unilateral ureteral obstruction. J. Vis. Exp. ...

Unilateral ureteral obstruction, or UUO, is a surgical procedure that involves the obstruction of one of the ureters, resulting in the impairment of urine flow from the kidney. To begin, prep an anesthetized male mouse in the supine position. Next, make a surgical incision in the abdominal skin.&#160;&#160;
Dissect the abdominal muscles and peritoneal membrane from the bladder to the lower-left edge of the ribs to expose the abdominal cavity. Gently displace the intestines to reveal the underlying left ureter.
The ureter is a narrow muscular tube that connects the bladder and the kidney and carries urine from the kidney to the bladder. There are two ureters, one connected to each kidney.
Now, surgically tie up the ureter at two regions, sufficiently apart. Cut the ureter between the two sutures to isolate the bladder from the ureter. Subsequently, place the intestines back into the abdomen and suture the incision.
Allow the mouse to recover. Within days of the procedure, obstruction of the left ureter leads to the accumulation of urine in the left kidney and results in the swelling of the kidney.&#160;

To perform the unilateral ureteral obstruction or UUO, make an incision in the skin at the abdomen and cut the muscle and peritoneal membrane. Place a 2.5-milliliter syringe underneath the mouse. Moisten two cotton swabs with PBS. Then, pull the intestines carefully to the side with the tweezers and place the swabs to identify the left ureter.
Use the tweezers to lift the left kidney. Use 4-0 silk to ligate the left ureter in two places approximately 1 centimeter apart. Cut the ureter at the center point of the two ligations and then use 4-0 nylon sutures to close the peritoneal membrane and incision.

unilateral-ureteral-obstruction-murine-model-surgical-procedure-to

Research

JoVE Encyclopedia of Experiments

Cancer Research

Urinary Tract Cancer

In vivo studies

6.7K Views. Unilateral ureteral obstruction, or UUO, is a surgical procedure that involves the obstruction of one of the ureters, resulting in the impairment of urine flow from the kidney. To begin, prep an anesthetized male mouse in the supine position. Next, make a surgical incision in the abdominal skin.   Dissect the abdominal muscles and peritoneal membrane from the bladder to the lower-left edge of the ribs to expose the abdominal cavity. Gently displace the intestines to reveal th...

Unilateral Ureteral Obstruction Murine Model: A Surgical Procedure to Obstruct the Urinary Flow from a Kidney in Mouse Models

In This Article

Abstract

Protocol

Disclosures

Tags