Intravenous Microinjection of Nephrotoxins into Zebrafish Embryo: A Technique to Deliver Nephrotoxic Agents into the Bloodstream of Zebrafish Embryo

Abstract

Source: McKee, Robert A. et al. Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury. J. Vis. Exp. (2016)

This video demonstrates the intravenous microinjection of nephrotoxins into the tail vein of a zebrafish embryo to study their chemical effects on renal function.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of Solutions

1. Make a 50x stock solution of E3 embryo media by mixing 73.0 g NaCl, 3.15 g KCl, 9.15 g CaCl₂, and 9.95 g MgSO₄ in 5 L of distilled water, and store at RT.
2. Make a 50x stock solution of E3 embryo media by mixing 73.0 g NaCl, 3.15 g KCl, 9.15 g CaCl₂, and 9.95 g MgSO₄ in 5 L of distilled water, and store at RT.
3. For culturing of zebrafish embryos, dilute the 50x stock solut....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Sodium Chloride	American Bioanalytical	AB01915
Potassium Chloride	American Bioanalytical	AB01652
Magnesium Sulfate	Sigma-Aldrich	M7506
Calcium Chloride	American Bioanalytical	AB00366
N-Phenylthiourea (PTU)	Aldrich Chemistry	P7629
Ethyl 3-aminobenzoate (Tricaine)	Fluka Analytical	A5040
Borosilicate glass	Sutter Instruments Co.	BF100-50-10
Flaming/Brown Micropipette puller	Sutter Instruments Co.	Mo. P097
UltraPure Agarose	Invitrogen	15510-027
Falcon Diposable Petri Dishes, Sterile, Corning:
60 mm x 15 mm	VWR	25373-085
100 mm x 15 mm	VWR	25373-100
Microinjection tray 150 mm x 15 mm	VWR	25373-187
Low Temperature Incubator	Fischer Scientific	11 690 516DQ
Micro Dissecting Tweezer	Roboz Surgical Instruments Co.	RS-5010
Micrometer	Ted Pella, Inc.	2280-24