Bacterial Endophthalmitis Mouse Model: A Method for Generating Bacterial Endophthalmitis via Intravitreal Injection of Bacterial Suspension into Eye of Mouse

Abstract

Source: Mursalin, M. H. et al., Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis. J. Vis. Exp. (2021).

This video describes a method for generating a mouse model that mimics bacterial endophthalmitis – an intraocular infection resulting from a bacterial invasion into the eye’s vitreous cavity – via intravitreal injection of a bacterial suspension into the eye. This mouse model helps study the disease mechanism and evaluate therapeutics for it.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Sterile glass needles

1. Turn On the needle pipette puller.
2. Adjust the Heater knob until the display shows 12.6.
3. Open the door and manually feed the 5 μL capillary tube through the upper clamp and heater filament until half of the tubing extends below the filament (Figure 1A).
4. Confirm that the capillary tube is in the “V” groove in the upper clamp. Tighten the upper clamp.
5. With th....

Results

Figure 1: Making beveled glass needles. Glass needles were made from disposable microcapillary pipettes using a needle/pipette puller and a micropipette beveler. (A) Clamped glass capillary tube in the needle/pipette puller. (B,C

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Cell Microinjector 	MicroData Instrument, Inc. 	PM2000 	NA
Microcapillary Pipets 5 Microliters 	Kimble 	71900-5 	NA
Micro-Pipette Beveler	 Sutter Instrument Co. 	BV-10	NA
Microscope OPMI Lumera 	Zeiss 		NA
Reverse action forceps 	Katena 	K5-8228	NA