Microinjector Use
Microinjector Use - Related Videos
02:24
Microinjection-Based Delivery of a Therapeutic Agent into an Awake Rodent Brain
0 Views •
2025
Source: Poland, R. et al. Rodent Brain Microinjection to Study Molecular Substrates of Motivated Behavior. J. Vis. Exp. (2015)In this video, a water-filled microinjection setup consisting of a syringe and microinjector tubing is used for precise drug delivery. A rat with a pre-implanted guide cannula undergoes targeted brain microinjection, enabling accurate administration of therapeutic agents in awake animals with minimal tissue damage.
03:20
Microinjection of Tetrodotoxin into the Brain of an Awake Female Rat to Inhibit Ovulation
0 Views •
2025
Source: Silva, C. et al. Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections. J. Vis. Exp. (2020)This video demonstrates the protocol for microinjection of tetrodotoxin (TTX) into the hypothalamus of a freely moving rat to transiently inhibit neuronal activity, suppress gonadotropin-releasing hormone secretion, and prevent ovulation.
03:57
Bacterial Endophthalmitis Mouse Model: A Method for Generating Bacterial Endophthalmitis via Intravitreal Injection of Bacterial Suspension into Eye of Mouse
0 Views •
2025
This video describes a method for generating a mouse model that mimics bacterial endophthalmitis – an intraocular infection resulting from a bacterial invasion into the eye’s vitreous cavity – via intravitreal injection of a bacterial suspension into the eye. This mouse model helps study the disease mechanism and evaluate therapeutics for it.
08:12
Zebrafish Microinjection Techniques
0 Views •
2023
One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved either through direct injection into the blastomere, or via natural cytoplasmic movements that occur after a...
02:33
A Technique to Develop a Parasite Infection in Intestinal Organoids via Microinjection
0 Views •
2025
This video demonstrates a microinjection technique to develop a human intestinal organoid infection model. The infectious sporozoite stage of the parasite Cryptosporidium parvum is microinjected into the organoid lumen. Upon invading the epithelial cells of the organoid, it undergoes asexual and sexual reproduction to complete its life cycle and spreads the infection.
FAQs
Delivery depends on coordinating two controls: a micromanipulator positions the fine glass micropipette, while applied pressure or mechanical displacement expels the selected fluid volume. This separation of positioning and ejection helps place material into a particular cell, embryo, or microscopic structure rather than releasing it broadly. The result is controlled manipulation at a defined target.
Compared with bulk delivery, Microinjector Use limits the treated volume and location. The micropipette can cross a target membrane and introduce material into a selected cell or intracellular compartment, allowing researchers to distinguish effects caused by defined delivery from effects of exposing a larger population or surrounding medium. This spatial control is central to cell-function studies.
The destination matters because microinjection can place reagents directly into defined compartments rather than only into the general environment of a cell. That capability supports experiments asking how a particular cell or compartment responds to DNA, RNA, proteins, dyes, or other reagents. It therefore links the physical act of injection to questions about intracellular function.
Different payloads support different experimental purposes. DNA and RNA enable gene-delivery studies, proteins and other reagents support direct manipulation, and dyes enable intracellular labeling. The method is therefore not limited to one class of material; investigators select the introduced substance according to whether they want to alter biological information, examine cell behavior, or mark a defined location.
A typical workflow begins by loading the chosen fluid into a fine glass micropipette, positioning the pipette with a micromanipulator, and bringing its tip to the selected biological structure. The tip then penetrates the target membrane, after which pressure or mechanical displacement expels a measured volume. This sequence combines placement, penetration, and controlled release.
Researchers choose this approach when an experiment requires direct, localized manipulation that bulk methods cannot provide. Biology applications include gene delivery, embryo manipulation, intracellular labeling, and cell-based studies of function. The technique is especially relevant to developmental biology, transgenesis, and experimental disease models, where the identity and location of the introduced material matter.