Bipolar Radiofrequency-Based Hemorrhage Arrest in Solid Organ Injury: A Technique to Arrest Bleeding in Porcine Model With Liver Injury Using Electrosurgery

Abstract

Source: Clements, T. W., et al. A Saline/Bipolar Radiofrequency Energy Device As an Adjunct for Hemostasis in Solid Organ Injury/Trauma. J. Vis. Exp. (2020).

This video demonstrates a technique of bipolar radiofrequency energy-based transcollation for hemorrhage arrest in solid organ injury in a porcine model. This method is helpful for the surgical management of traumatic injury by achieving hemostasis by arresting bleeding at the injury site.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Model Preparation

1. House the 50 kg adult male pig in an animal care facility for 1 week prior to the surgery to acclimatize the animal to the housing conditions and the handlers. Fast the model for a minimum of 6 h prior to the initiation of anesthesia.
2. Anesthetize the model using an intramuscular injection of ketamine (33 mg/kg), atropine (0.04 mg/kg), and buprenorphine (0.05 mg/kg) as well as inhaled isoflurane (5%)⁹.
3. Move the model into the supine pos....

Results

Figure 1: Saline/bipolar radiofrequency (SBRF) energy device. (A) This panel shows the SBRF device handpiece with the single-button design. (B) This panel shows the SBRF's 6.0 blunt bipolar sealing tip.

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Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Aquamantys pump generator	Medtronic	40-402-1
Aquamantys 6.0 bipolar sealer	Medtronic	23-112-1
Electrosurgical pencil with tip	Megadyne	39
Porcine animal
Porcine ventilator/induction and anesthetic medications
2 x 1 liter bags of 0.9% normal saline
2 x scalpels (#10)
Belfour abdominal retractor
Suction tubing
Suction tip
Suction device/wall connector
Suction canister
Debakey forceps
Metz scissors
Curved Mayo scissors
Closing suture (1-0 Nylon)
20 x Laparotomy sponges
2 x Kelley clamps
2 x snap clamps