Exposing Infrarenal Abdominal Aorta in Porcine Model: A Surgical Procedure to Access Porcine Infrarenal Abdominal Aorta for Further Studies

Abstract

Source: Shannon, A. H. et al., Porcine Model of Infrarenal Abdominal Aortic Aneurysm. J. Vis. Exp. (2019)

This video demonstrates the surgical procedure to locate and dissect the infrarenal aorta from the surrounding tissues for accessing it. The exposed aorta can be used for further studies.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Surgical technique

1. Perform sterilization of the surgical area using sterile gauze, povidone-iodine and 70% isopropyl alcohol. Drape the pig in the usual sterile fashion. Take a blood sample prior to incision. 
   NOTE: At this point, all equipment, including instruments, balloons, wires, etc. must be sterile.
2. Using an eleven blade or Bovie electrocautery, perform a midline laparotomy to enter the abdominal cavity.
3. Displace the abdominal v....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Bovie electrocautery	Bovie Medical	A2350
DeBakey Atraumatic Forceps	MFI medical	52-4977
Metzenbaum Scissors	MFI medical	61-0004
Mixter Forceps, Standard Grade, Right angle	Cole-Parmer	UX-10818-16