Impedance-Based Cellular Assay to Measure Multiplicity of Infection of Influenza Virus

Abstract

Source: Labadie, T., et al., Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis. J. Vis. Exp. (2021)

In this video, we discuss the correlation between the CIT₅₀ and the multiplicity of viral infections. The virus-infected cells display cytopathic effects, causing their death and detachment from the gold microelectrode, which in turn, reduces the impedance.

Protocol

1. Correlation between CIT₅₀ values and multiplicity of infection

1. Add 100 µL of sterile 1x MEM culture medium in each well of the E-plate. Insert the E-plate into the cradle pocket of the instrument at 35°C.
2. Measure the background as described:
3. Open the software.
   1. In "Default experiment pattern setup", choose the selected cradle(s) and double-click on the top page, then enter the name of the experiment. Click "Layout" and enter the necessary sample information for each selected well of the plate; then, click "Apply" when finished. Click "Schedule....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
E-Plate 16 (6 plates)	ACEA Biosciences, Inc	5469830001	E-plates are available in different packaging
MEM 1X	Life technologies (gibco)	31095029
TPCK-Trypsin	Worthington	LS003740
xCELLigence Real-Time Cell Analysis Instrument S16	ACEA Biosciences, Inc	380601310	The xCELLigence RTCA S16 instruments are available in different formats (16-well, 96-well, single or multi-plate)