Diffuse Correlation Spectroscopy to Assess Cerebral Blood Flow in a Mouse Model

In This Article

Abstract

Source: Brothers, R. O., et al. Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury. J. Vis. Exp. (2022)

In this video, we demonstrate the diffuse correlation spectroscopy technique to measure cerebral blood flow in a mouse model of mild traumatic brain injury.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board

1. Assessment of cerebral blood flow with diffuse correlation spectroscopy

DCS data acquisition
1. Remove hair on the scalp. Because DCS works best in the absence of hair, it is necessary to remove fur on the head prior to the start of experiments. Typically, hair removal is done 1-3 days prior to the start of the study.
  1. Induce mice with 4.5% isoflurane in 100% oxygen for 45 seconds and maintain with 1-2% isoflurane in 100% oxygen.....

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Results

Optical sensor and anesthesia setup; mouse imaging, source-detector pattern; diagram.
Figure 1: Measurement of cerebral blood flow by Diffuse Correlation Spectroscopy. (A) An optical sensor is gently manually held over the right hemisphere to measure blood flow in an anesthetized mouse. (B) Representative sensor placement on the right .......