Hematoxylin and Eosin Staining to Assess Colon Damage in DSS-Induced Colitis

Abstract

Source: Du, Y., et al. Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model. J. Vis. Exp. (2018)

This video demonstrates the hematoxylin and eosin (H&E) staining method to evaluate colon damage in a murine model of DSS-induced colitis — an inflammatory bowel disease. Upon isolating the colon from a DSS-treated mouse and obtaining sections of the tissue, H&E staining of the sections helps identify the inflammation-mediated tissue damage.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of Mice and DSS

1. Keep the knockout (α-gustducin^-/-) mice and age-, gender-, and body weight-matched wild-type control (α-gustducin^+/+) C57BL/6 mice individually in clean cages.
   NOTE: The knockout mice have been backcrossed with C57BL/6 mice for over 20 generations and have a nearly 100% C57BL/6 genetic background.
2. Dissolve 30 g of dextran sulfate sodium (DSS) powder in 1 L of autoclaved wate....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Dextran Sulfate Sodium Salt (DSS)	MP Biomedicals	2160110
H&E Staining Kit	BBI Life Sciences	E607318
Phosphate-Buffered Saline (PBS)	Sangon Biotech	B548117
BD 10 ml Syringe	BD Biosciences	309604
Instruments and equipment
Balance
Scissors
Forceps
Staining jars
Software
Imag-Pro Plus	Media Cybernetics, Inc.