Convection-Enhanced Delivery of Antibodies into a Mouse Brain

In This Article

Abstract

Source: Beffinger, M., et al. Delivery of Antibodies into the Murine Brain via Convection-enhanced Delivery. J. Vis. Exp. (2019).

This video demonstrates a convection-enhanced delivery (CED) technique for antibody delivery into the mouse brain. The skull of an anesthetized mouse is exposed, and a burr hole is created at the target area for antibody delivery. A step catheter containing antibodies is inserted at the target site, and via an infusion pump, a pressure gradient is created to deliver antibodies at a controlled rate.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of the Step Catheters

Preparation of a fused silica tubing for the step of the catheter
1. Cut the fused silica capillary with an inner diameter of 0.1 mm and wall thickness of 0.0325 mm tubing to a length of 30 mm.
2. Examine the tubing for cracks and heat polish the ends using a microforge to ensure the tubing openings have a smooth surface.
Fixation of the inner tube in a metal needle

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Results

Adhesive application diagram, showing 10 mm sections for structural assembly process.
Figure 1: A schematic drawing depicting the application area of the adhesive. The upper 10 mm of the fused silica tubing is inserted into the metal needle. Apply the glue on the 10 mm tubing starting from the tip of the metal needle.

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