Assays for Qualitative and Quantitative Assessment of Mouse Urine Glucose Concentrations

Abstract

Source:
Tumininu S. Faniyan¹, Dennis J. Samuel¹, Kavaljit Chhabra<sup>1

1</sup>University of Rochester Medical Center.

This video demonstrates a procedure for collecting mouse urine and assessing its glucose levels. To evaluate the glucose level, immerse a test strip coated with enzymes and a chromogenic dye into the sample, initiating a color change that indicates the glucose levels. For quantifying urine glucose levels, introduce a urine sample to a multiwell plate with escalating glucose concentrations, add the reaction mixture, and incubate. Measure the absorbance to create a standard curve and compute the glucose levels based on the sample absorbance.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Spot urine glucose test

1. Place a piece of clean Parafilm on a flat surface.
2. Gently pick up a mouse by the tail and set it on the Parafilm.
3. Restrain the mouse by the scruff using the thumb and index finger. Gently pick up the mouse while restrained at the scruff, causing the mouse to urinate as a stress response. Allow for a drop of urine to fall onto the parafilm surface. Return the mouse to its cage after collecting the urine drop.
4. Dip t....