A Technique to Generate a Novel Delivery Platform for a Delayed Burst Release of Molecules

Abstract

Source: Arun Kumar, S. et al., Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform. J. Vis. Exp. (2020)

This video demonstrates a method for the generation of a polymer bubble or polybubble-based novel platform that enables delayed burst release. Polyester-based polymers are used to form the polybubbles with core-shell structure, and small molecules and antigens can be used as cargo.

Protocol

1. Polycaprolactone triacrylate (PCLTA) synthesis

1. Dry 3.2 mL of 400 Da polycaprolactone (PCL) triol overnight at 50 °C in an open 200 mL round bottom flask and potassium carbonate (K₂CO₃) in a glass vial at 90 °C.
2. Mix the triol with 6.4 mL of dichloromethane (DCM) and 4.246 g of K₂CO₃ under argon.
3. Mix 2.72 mL of acryloyl chloride in 27.2 mL of DCM and add dropwise to the reaction mixture in the flask over 5 min.
4. Cover the reaction mixture with aluminum foil and leave it undisturbed at room temperature for 24 h under argon.
5. After 24 h, filter the r....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
1-Step Ultra Tetramethylbezidine (TMB)-Enzyme-Linked Immunosorbent Assay (ELISA) Substrate Solution	Thermo scientific	34028
2-Hydroxy-2-methylpropiophenone	TCI AMERICA	H0991
450 nm Stop Solution for TMB Substrate	Abcam	ab17152
Acryloyl chloride	Sigma Aldrich	A24109-100G
Acriflavine	Chem-Impex International	22916
Anhydrous ethyl ether	Fisher Chemical	E138-500
Anti-HIV1 gp120 antibody conjugated to horseradish peroxidase (HRP)
Bovine serum albumin (BSA)	Fisher BioReagents	BP9700100
BSA-CF488 dye conjugates	Invitrogen	A13100
Carboxymethylcellulose (CMC)	Millipore Sigma	80502-040
Chloroform	Fisher Chemical	C2984
Coating buffer	Abcam	ab210899
Dichloromethane (DCM)	Sigma Aldrich	270997-1L
Diethyl ether	Fisher Chemical	E1384
Legato 100 Syringe Pump	KD Scientific	14 831 212
Nonfat dry milk	Andwin Scientific	NC9022655
Potassium carbonate	Acros Organics	AC424081000
Phosphate saline buffer	Fisher BioReagents	BP3991
(Poly(caprolactone)	Sigma Aldrich	440744-250G
(Poly(caprolactone) triol	Acros Organics	AC190730250
Poly (lactic-co-glycolic acid) diacrylate	CMTec	280050
Recombinant HIV1 gp120 + gp41 protein	Abcam	ab49054
Trehalose	Acros Organics	NC9022655